EphB2蛋白在胰腺癌组织中的表达及其临床意义  

Expression and Clinical Significance of EphB2 Protein in Human Pancreatic Cancer

作　　者：田海军[1] 马凯[2] 张绍军[1] 

机构地区：[1]沈阳医学院奉天医院普外一科,辽宁沈阳110024 [2]中国医科大学盛京医院第一微创外科

出　　处：《沈阳医学院学报》2009年第3期159-161,共3页Journal of Shenyang Medical College

摘　　要：目的:探讨EphB2蛋白在胰腺癌中的表达同肿瘤临床病理特征之间的关系。方法:选取中国医科大学盛京医院病理科2003年2月至2006年2月期间外科手术切除胰腺癌标本24例,胆管下段癌行胰十二指肠切除术而切除的正常胰腺组织6例,应用免疫组化方法,检测胰腺癌组织及正常胰腺组织标本中EphB2蛋白的表达情况。结果:EphB2蛋白表达免疫组织化学检测结果显示,阳性染色主要定位于细胞胞浆,呈棕黄色或棕褐色。EphB2蛋白在胰腺癌组织中表达阳性率为66.67%(16/24),评分值为3.08±1.349,明显高于正常组织中的表达16.67%(1/6),评分值为1.17±1.169,差异有统计学意义(P<0.01)。在胰腺癌各病理分级组之间的差别无统计学意义(P>0.05)。淋巴结转移组的EphB2蛋白表达高于淋巴结未转移组,差别有统计学意义(P<0.05)。结论:EphB2蛋白可能与胰腺癌的发生发展相关。且可能参与了胰腺癌的转移。Objective： To analyze biological significance of EphB2 protein in pancreatic cancer, and provide evidences for the gene therapy of pancreatic cancer in future. Method： The 24 specimens of pancreatic cancer were selected from Feberary, 2003 to Feberary, 2006 at the Shengjing Hospital affiliated to China Medical University, and 6 specimens of normal pancreatic tissue were collected as negative control. The expression of EphB2 in the tumor and normal tissue were detected with immunohistochemistry methods. Results： Cells staining for EphB2 showed yellow or brown granules in cytoplasm which mainly distribute in carcinoma cells and less present in normal tissues and tissues surrounding carcinoma. EphB2 positive rate （66. 67% , 16/24） and EphB2 score （3.08 ±1. 349） of pancreatic cancer group were obviously higher than control group （ 16. 67%, 1/6） and EphB2 score （ 1.17 ±1. 169） （P 〈 0. 01 ）. But there were no significant differences between patients with high, middle and low differentiated carcinoma （P 〉 0. 05 ）. EphB2 expression in the group with lymphatic metastasis was significantly higher than that without lymphatic metastasis （P 〈 0. 05 ）. Conclusion： EphB2 may have relationship with the genesis and development of pancreatic cancer. And it suggested that there is the association between EphB2 expression and lymphatic metastasis in pancreatic cancer and that EphB2 may be able to promote the metastasis of tumor cells in pancreatic cancer.

关 键 词：胰腺癌 EphB2蛋白 淋巴结转移 免疫组化 

分 类 号：R735.9[医药卫生—肿瘤]