反复胰蛋白酶消化法培养SD大鼠视网膜Muller细胞  

Repeated Pancreatic Enzyme-degisting Method for Culturing Muller Cells of SD Rats

作　　者：王若冰[1] 

机构地区：[1]上海第二军医大学附属一院眼科,上海200433

出　　处：《沈阳医学院学报》2009年第3期171-173,共3页Journal of Shenyang Medical College

摘　　要：目的:通过反复胰蛋白酶消化法,建立Muller细胞的培养和纯化方法。方法:采用新生7天的SD大鼠,解剖镜下取视网膜,反复胰蛋白酶消化法消化贴壁后的细胞,细胞形态一致后行免疫组织化学和形态学检查。结果:所得细胞的90%左右谷氨酰胺合成酶(GS)免疫组化染色阳性;荧光显微镜下可见细胞胞体巨大,足突明显,证明是Muller细胞。结论:反复胰蛋白酶消化法是一种简单、可靠的Muller细胞培养方法。Objective： To find a way to culture and purify Muller cells by series of steps. Method： Retinas of 7-day-newborn rats, eyeballs were required under anatomical microscope, repeated pancreatic enzyme digesting after cells were attached to nutritive medium. Muller cells were identified by immunocytochemitry, and the morphology observed by a fluorescence microscope. Results： Cells cultured have large cell bodies and more than one foot processes. About 90% cells are positive staining for glutamine synthetase, proved to be Muller cell. Conclusion： Repeated pancreatic enzyme-digesting method is a simple and reliable way for culturing Muller cells.

关 键 词：MULLER细胞 细胞培养 胰蛋白酶消化法 

分 类 号：R774[医药卫生—眼科]