溶菌酶和乳铁蛋白在慢性鼻窦炎钩突粘膜中的表达  

Lysozyme and lactoferrin in human uncinate process mucosa during chronic sinusitis

作　　者：张罗[1,2] 韩德民[1,2] 王植平 张勇[1,2] 周兵 刘华超[1,2] 范尔钟 李颍[1,2] 

机构地区：[1]首都医科大学附属同仁医院耳鼻咽喉 [2]北京市耳鼻咽喉科研究所

出　　处：《中华耳鼻咽喉科杂志》1998年第4期219-221,共3页Chinese Journal of Otorhinolaryngology

摘　　要：目的了解钩突在局部防御慢性炎症过程中的作用。方法采用免疫组化ＡＢＣ法，检测溶菌酶（ｌｙｓｏｚｙｍｅ，ＬＺ）和乳铁蛋白（ｌａｃｔｏｆｅｒｉｎ，ＬＦ）在１７侧健康和７０侧慢性鼻窦炎患者（分为单纯炎症组和合并鼻息肉组）钩突粘膜中的表达情况。结果①ＬＺ在单纯炎症组钩突粘膜杯状细胞中表达较健康对照组加强（Ｐ＜０．０５）；ＬＦ在健康和慢性鼻窦炎钩突杯状细胞中常为阴性表达；②ＬＺ在健康和慢性鼻窦炎钩突粘膜腺体的浆液性和粘液性细胞中均有表达；ＬＦ只在浆液性腺细胞胞浆中表达。合并鼻息肉组钩突粘膜腺细胞中ＬＺ和ＬＦ的表达增强，其ＬＺ的表达与健康对照组和单纯炎症组相比差异均有显著性（Ｐ＜０．０１，Ｐ＜０．０５），ＬＦ的表达与健康对照组比较差异有非常显著性（Ｐ＜０．０１）；③钩突粘膜上皮细胞对ＬＺ和ＬＦ多为阴性表达。结论钩突粘膜中的杯状细胞和混合腺对ＬＺ和ＬＦ的分泌起重要作用，可能参与构成局部免疫防御系统。Objective To study the local protective role of lysozyme(LZ) and lactoferrin(LF) in the uncinate process mucosa during chronic sinusitis. Methods Expression of LZ and LF was determined in 17 samples from normal subjects and 70 samples from chronic sinusitis patients with ABC immunohistochemical method. According to the presence or absence of nasal polyps, patients were divided into two groups. Results Serous cells of submucosal glands displayed a strongly positive staining reaction to both LZ and LF in the normal uncinate process mucosa and mucosa from patients with chronic sinusitis. A positive though weak staining for LZ could also be found frequently within mucous cells of submucosal mixed glands and occasionally within goblet cells. In the mucosa from patients without nasal polyps,the staining reaction to LZ appeared to be intensified in goblet cells when compared with normal controls (P<0.05). In patients with nasal polyps,the staining reaction to LZ appeared to be intensified in submucosal glands when compared with normal controls (P<0.01) and patients without nasal polyps (P<0.05). For LF,the staining reaction from patients with nasal polyps was stronger than that in normal controls (P<0.01).The epithial cells stained negatively for LZ and LF. Conclusions It suggests that the observed increase in LZ and LF secreting activity of goblet cells and submucosal mixed glands may play a part role in the defense mechanism of uncinate process mucosa during the course of chronic sinusitis.

关 键 词：鼻窦炎 溶菌酶 乳铁蛋白 免疫组化 

分 类 号：R765.410.2[医药卫生—耳鼻咽喉科]