人疱疹病毒6型对EB病毒复制影响的研究  

Study on the effect of human herpesvirus 6 on replication of EpsteinBarr virus

作　　者：夏青生[1,2,3] 孟广明[1,2,3] 艾彦红 沐桂藩[1,2,3] 

机构地区：[1]青岛市立医院耳鼻咽喉科 [2]北京协和医院耳鼻喉咽科 [3]中国医科院基础医学研究所微生物免疫室

出　　处：《中华耳鼻咽喉科杂志》1998年第4期235-236,共2页Chinese Journal of Otorhinolaryngology

摘　　要：目的探讨人疱疹病毒６型（ＨＨＶ６）对ＥＢ病毒（ＥＢＶ）复制的影响。方法用ＨＨＶ６感染Ｒａｊｉ细胞和ＥＢＶ转化的淋巴细胞（ＬＣＬ），并用抗ＨＨＶ６单克隆抗体（ＭＡｂ）作免疫萤光分析（ＩＦＡ）确定ＨＨＶ６已感染这二种细胞。用抗ＥＢＶ人血清作ＩＦＡ观察ＥＢＶ抗原表达。结果ＨＨＶ６感染Ｒａｊｉ细胞后可见细胞病变效应（ＣＰＥ）；而ＬＣＬ未见ＣＰＥ。这二种细胞株用抗ＨＨＶ６的ＭＡｂ作ＩＦＡ均检出ＨＨＶ６，而对照组未检出ＨＨＶ６；用抗ＥＢＶ人血清作ＩＦＡ，感染ＨＨＶ６的二种细胞中均检出ＥＢＶ抗原。结论ＨＨＶ６促进了ＥＢＶ抗原表达，并提示在鼻咽癌的发生中ＨＨＶ６和ＥＢＶ可能起协同致病作用。Objective To examine the effect of human herpesvirus 6(HHV6) on the replication of EpsteinBarr virus (EBV) in cell lines. Methods Both EBVinfected Raji cells and EBV transformed lymphoblastoid cell lines (LCL) were infected with HHV6. Immunofluoresence assay (IFA) with monoclonal antibodies (MAb) against HHV6 was applied to confirm the infection of HHV6 in the two cell lines. Expression of EBV antigen was examined by IFA using human antiEBV serum. Results Following HHV6 infection, cytopathic effects (CPE) were observed in Raji cells but not in LCL. HHV6 were detected in both cell lines by IFA with antiHHV6 MAb, but not in controls. EBV antigens were detected by IFA with human serum against EBV in both HHV6 infected cell lines. Conclusion Data in this study suggest that HHV6 promotes the expression of EBV antigen and may contribute to the pathogenesis of nasopharyngeal carcinoma in cooperation with EBV.

关 键 词：鼻咽肿瘤 疱疹病毒 病毒复制 

分 类 号：R739.630.2[医药卫生—肿瘤]