水貂12S rRNA基因序列测定及鼬属动物的系统进化关系  

Determination of Mitochondrial 12S rRNA Gene Sequence of Mink and Phylogeny of the Mustela

作　　者：涂剑锋[1] 王雷[1] 荣敏[1] 徐佳萍[1] 章秀婷[1] 杨福合[1] 

机构地区：[1]中国农业科学院特产研究所,吉林132109

出　　处：《经济动物学报》2009年第3期150-154,共5页Journal of Economic Animal

基　　金：国家科技支撑计划项目(2006BAD06B06)

摘　　要：参照紫貂线粒体基因组序列设计引物,通过PCR直接测序的方法获得7个水貂品种线粒体12SrRNA基因全序列。分析显示:水貂12S rRNA基因的全长为960或962 bp,平均碱基组成为37.50%A、17.60%G、22.19%T和22.71%C,检测到8个多态位点,转换与颠换的比值为3∶1。结合GenBank检索得到的11种鼬属动物同源序列,以紫貂为外群,分别用邻接法和最大简约法构建鼬属动物系统进化树,两种进化树的拓扑结构基本一致。结果表明:我国水貂与美洲水貂亲缘较近,与欧洲水貂亲缘较远。In this study, we designed primers according to sable gene and obtained the complete mitochondrial 12S rRNA gene sequences of 7 breeds of mink by PCR amplification, sequencing and assembling. Sequence analysis revealed that 12S rRNA gene of minks was 960 or 962 bp in length, the composition of the nucleotides was 37.50% A, 17.60% G, 22.19% T and 22.71% C. 8 variable sites were observed, and the transition/transversion（Ts/Tv）was 3. Given sable as outgroup and homologous sequences of 11 species in the mustela retrieved from GenBank, the phylogenetic trees were constructed respectively by N- J and MP methods. The results indicated that minks in China compared with American minks had closer relationship than that with European mink.

关 键 词：水貂 12S RRNA基因 系统进化关系 

分 类 号：S865.22[农业科学—野生动物驯养]