人鼻咽癌CNE-2细胞转染方法的比较研究  

Comparative study of transfection of DNA to human nasopharyngeal carcinoma CNE-2 cells

作　　者：温见翔[1] 余振东[1] 吴波[1] 黄达娜[2] 

机构地区：[1]北京大学深圳医院,广东深圳518036 [2]深圳市疾病预防控制中心,广东深圳518020

出　　处：《中国实验诊断学》2009年第9期1140-1141,共2页Chinese Journal of Laboratory Diagnosis

基　　金：广东省科技计划资助项目(2008B030301280);深圳市科技计划资助项目(200802007)

摘　　要：目的比较三种非病毒载体转染方法转染人鼻咽CNE-2细胞。方法分别采用脂质体转染法、电穿孔转染法、磷酸钙纳米颗粒转染法将pEGFP-N1质粒DNA转染人鼻咽癌CNE-2细胞,转染24 h后荧光显微镜观察EGFP的表达,计算转染率;MTT实验比较各转染方法对CNE-2细胞的细胞毒性。结果脂质体转染组的转染率为(51.9±2.8)%;电穿孔转染组的转染率为(42.8±2.6)%;磷酸钙纳米颗粒转染组的转染率为(48.1±2.3)%。经统计学分析,三种方法转染CNE-2细胞的转染率相互之间有显著的差异(P<0.001);MTT结果显示,磷酸钙纳米颗粒转染法的细胞毒性最小。结论磷酸钙纳米颗粒转染法转染效率相对较高,而细胞毒性小,可作为转染CNE-2细胞的首选转染方法。Objective To compare the effective of tmnsfection methods of nonviral vectors. Methods The plasmid pGEFP-N1 was conducted into human nasopharyngeal carcinoma CNE-2 cells respectively by liposome, electroporation and calcium ＇phosphate nanoparticles. The expression of EGFP was observed by fluorescence microscope 24 hours later after transfection. MTT assay was used to observe the cytotoxicity of the vectors. Results The transfection efficiency of liposome, electroporation and calcium phosphate nanopartieles was （51.9 ± 2.8） %, （ 42.8 ± 2.6 ） % and （ 48.1 ± 2.3 ） % separately with the difference being statistically significant （ P 〈 0. 001 ）. It was proved that the cytotoxicity of calcium phosphate nanoparticles was least by MTT assay. Conclusion calcium phosphate nanopartieles could be the preferred nonviral vector to transfecting CNE-2 cells.

关 键 词：CNE-2细胞 脂质体 电穿孔 磷酸钙纳米颗粒 

分 类 号：R739.63[医药卫生—肿瘤]