实时定量PCR检测原发性胆汁性肝硬化单核细胞中Siglec-1 mRNA的表达  

Expression of siglec-1 mRNA in monocytes from patients with primary biliary cirrhosis assayed by FQ-PCR

作　　者：周晔[1] 蒋廷旺[1] 谷明莉[1] 陈燕[1] 奚伟强[1] 姚定康[1] 邓安梅[1] 仲人前[1] 

机构地区：[1]第二军医大学长征医院实验诊断科,上海200003

出　　处：《中国实验诊断学》2009年第9期1190-1192,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金(30671840;30772017;30770997);上海市科委基金(08QH14001)资助

摘　　要：目的检测Siglec-1(sialic acid-binding immunoglobulin-like lectins,唾液酸结合的免疫球蛋白样凝集素,CD169)在原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者外周血单核细胞上的mRNA表达水平,并探讨其在原发性胆汁性肝硬化发生发展中的作用。方法实时荧光相对定量PCR方法检测30例原发性胆汁性肝硬化患者及30例健康对照者3、0例肝炎后肝硬化对照者外周血单核细胞中Siglec-1 mRNA的含量;生化常规测定所有入选者血清GGT、ALP指标水平。结果原发性胆汁性肝硬化组Siglec-1 mRNA的相对表达量为健康对照组的3.42倍,差异具有统计学意义(P<0.01)。PBC患者Siglec-1 mRNA增高与GGT(r=0.482,P<0.01)和ALP(r=0.365,P<0.05)密切相关。结论原发性胆汁性肝硬化患者单核细胞中mRNA含量显著增加,说明原发性胆汁性肝硬化患者外周血单核细胞已经发生巨噬细胞化,单核巨噬细胞介导的免疫炎症反应在原发性胆汁性肝硬化发生发展过程中起重要作用。Objective To study on expression of Siglec-1 mRNA in monocytes from patients with Primary Biliary Cirrhosis （PBC） .Methods Total RNA was extracted from monocytes from 30 patients with PBC,30 healthy controls and 30 disease（liver cirrhosis after HBV infection） controls. Real time Fluorescence Quantitative Polymerase Chain Reaction（FQ-PCR） was performed to assay the level of Siglec-1 mRNA. The levels of GGT and ALP in sera were evaluated by routine tests. Results Compared with normal controls, expression of Siglec- 1 mRNA increased significantly （ relative expression as 3.42 versus 1, P 〈 0. 01 ）. In addition, expression of Siglec-1 mRNA were related to the levels of GGT（ r = 0. 482, P 〈 0.01 ） and ALP（ r = 0. 365, P 〈 0.05）. Conclusion Inflammation mediated by mononuclear macrophage play important roles in the course of PBC.

关 键 词：原发性胆汁性肝硬化 SIGLEC-1 

分 类 号：R575.2[医药卫生—消化系统] R450[医药卫生—内科学]