HBV、HCV感染和XPC基因Ala499Val、Lys939Gln与肝癌关系的1：1匹配病例对照研究  

A 1：1 matched case-control study on the interaction between HBV, HCV infection and DNA repair gene XPC Ala499Val, Lys939Gln for primary hepatocellular carcinoma

作　　者：蔡旭玲[1] 郜艳晖[1] 余卓文[2] 吴兆权[2] 周卫平[1] 杨翌[1] 许雅[1] 宋韶芳[1] 陈思东[1] 

机构地区：[1]广东药学院公共卫生学院,广州510310 [2]佛山市顺德区慢性病防治中心

出　　处：《中华流行病学杂志》2009年第9期942-945,共4页Chinese Journal of Epidemiology

基　　金：基金项目：国家自然科学基金青年基金（30500421）

摘　　要：目的探讨环境因素HBV、HCV感染与DNA修复基因XPC第8外显子Ala499Val、第15外显子Lys939Gln交互作用在原发性肝癌（肝癌）发生中的作用。方法在广东省顺德地区采用1：1匹配的病例对照研究，PCR技术检测Ala499Val、Lys939Gln基因型，条件logistic回归分析环境与基因单独作用和交互作用对肝癌的影响。结果HBsAg阳性者中，无携带Ala499Val突变型基因者和至少携带一个突变型基因者的OR值分别为3．768（95％CI：1．137。12．485）和3．667（95％CI：1．122～11．981）；无携带Lys939Gln突变型基因者和至少携带一个突变型基因与肝癌关联的OR值分别为6．778（95％CI：2．025—22．688）和3．152（95％C1：1．062～9．351）。HCV感染者中，无携带Ala499Val突变型基因和至少携带一个突变型基因的OR值为2．955（95％CI：0．587。14．869）和1．085（95％C1：0．307～3．839）；携带至少一个突变型基因和无携带Lys939Gln突变型基因相比，OR值由4．197（95％CI：0．870～20．243）降低为0．887（95％CI：0．228—3．448）。但基于相乘模型的基因和环境交互作用均未达到统计学意义。结论携带Ala499Val突变型基因有降低HCV感染者的肝癌发病风险的趋势；而携带Lys939Gln突变型基因对HBV感染者和HCV感染者的肝癌发病风险都有降低的趋势，需要加大样本量进一步研究。Objective To evaluate the interaction between environmental factors, HBV/ HCV infections and DNA repair gene XPC exon 8 Ala499Val, exon 15 Lys939Gln on related risks to primary hepatocellular carcinoma （PHC）. Methods A 1 ： 1 matched ease-control study was conducted in Shunde city, Guangdong province. The genotypes of Ala499Val and Lys939Gln were detected by polymerase chain reaction restrictive fragment length polymorphism （PCR-RFLP） analysis, and gene-environment interactions were analyzed by conditional logistic regression. Results Among people infected by HBV with non- or at least one mutant gene of Ala499Val carriers, the risk of PHC significantly increased, with ORs as 3.768 （95% CI： 1.137-12.485） and 3.667 （95% CI： 1.122-11.981 ） respectively. With non- or at least one mutant gene of Lys939Gin, the risk was increasing with ORs as 6.778 （95% CI： 2.025-22.688） and 3.152 （95% CI： 1.062-9.351 ） respectively. In those with HCV infection, non- or at least one mutant gene of Ala499Val might increase the risk with ORs as 2.955 （95% CI： 0.587-14.869）, 1.085 （95% CI： 0.307-3.839） respectively. However, when compared to the ones with no mutant gene of Lys939Gln among the same research subjects, those carrying at least one gene may decrease the risk,with OR lowered ffom4.197（95%CI：0.870-20.243）to 0.887（95%CI： 0.228-3.448）. But the interactions between HBV infection, HCV infection and XPC genes were not statistically significant. Conclusion Among people infected by HCV, the mutant gene of Ala499Val had the tendency to lower the risk of PHC, and the mutant gene of Lys939Gln also appeared the same in the population with either HBV infection or HCV infection in Shtmde, Guangdong. Another study with large samples should be performed to analyze the interactions among environments-genes.

关 键 词：原发性肝癌 XPC基因多态性 乙型肝炎病毒感染 丙型肝炎病毒感染 交互作用 

分 类 号：R735.7[医药卫生—肿瘤]