半夏、水半夏RAPD分析及构建特异DNA探针  

RAPD Analysis and Construction of Specific DNA Probes between Pinellia ternata and Typhonium flagelliforme

作　　者：陈果[1] 白权[2] 王朝莉[1] 任碧轩[1] 唐恩洁[1] 冯莉[1] 敬保迁[1] 胡为民[1] 

机构地区：[1]川北医学院免疫与分子生物学研究所,四川南充637000 [2]川北医学院附属医院药剂科,四川南充637000

出　　处：《中药材》2009年第9期1374-1375,共2页Journal of Chinese Medicinal Materials

基　　金：四川省科技厅应用基础研究项目(04JY029-014-1)

摘　　要：目的:建立鉴定半夏、水半夏的分子标记方法。方法:利用22条随机引物,对四川两种半夏、广西两种水半夏基因DNA进行RAPD扩增,并对其差异条带进行测序、标记构建探针和Southern杂交。结果:半夏、水半夏基因DNA之间扩增的差异条带较多。通过测序、标记构建探针和杂交确认一半夏特异的标记分子。结论:通过RAPD和构建特异DNA探针能准确鉴别半夏和水半夏。Objective： To establish a molecular marking method to identify Pinellia ternata and Typhonium flagelliforme. Methods： Twenty-two random oligonucleotide primers were used in RAPD analysis on the genomic DNA of two types of Pinellia ternata in Sichuan and two types of Typhonium flagelliforme in Guangxi. The special fragments were sequenced, marked as probes and then conducted Southern blot. Results ： A great deal of special bands was found between Pinellia ternata and TyphoniumflageUiforme. A Pinellia terna- ta specific molecule was screened. Conclusion： RAPD analysis and specific DNA probes show potential value in the identification of PineUia ternata and Typhonium flageUiforme.

关 键 词：半夏 水半夏 RAPD技术 特异DNA探针 SOUTHERN杂交 

分 类 号：R282.5[医药卫生—中药学]