Cloning and Sequence Analysis of HN and F Protein Genes from a Strain of Goose Paramyxovirus  

鹅副粘病毒GX1株的HN和F蛋白基因的克隆和序列分析

作　　者：易春华[1] 潘杰[1] 付薇 颜健华 徐贤坤 熊毅 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西壮族自治区动物疫病预防控制中心,广西南宁530001

出　　处：《Agricultural Science & Technology》2009年第4期75-78,共4页农业科学与技术（英文版）

基　　金：Supported by the Development Program for Guangxi Science andTechnology(0719004-3G)~~

摘　　要：[ Objective] The study was to clone HN and F genes from GX1 strain of goose paramyxovirus and analyze their sequences. [ Method] According to the full nucleotide sequence of GPV-SF02 strain of goose paramyxovirus, two pairs of pdmers were designed to amplify the HN and F genes from GX1 strain of goose paramyxovirus isolated from diseased goose in Guangxi Zhuang Autonomous Region; the amplified products were ligated into pMD18-T vector and sequenced. [ Result ] HN and F genes of this strain tested were 1 716 and 1 662 bp in full nucleotide length, respectively; both showed the homologues of about 97.3% with GPV- SF02 strain, of 80.3% -97.5% with strains LaSota, F48E9 and JS, of just 84.8% with Miyadera strain. [ Conclusion] The results show that isolated strain BX1 matches to virulent APMV-1 strain, belonging to genotype Ⅶ of APMV-1 strain.1材料与方法1．1病毒来源鹅副粘病毒分离株GX1株由广西壮族自治区动物疫病预防控制中心实验室分离保存。1．2试剂及仪器各种PCR反应试剂、PMD-18T载体、胶回收试剂盒、限制性内切酶等均购自宝生物工程（大连）公司。仪器：恒温孵化箱、PCR反应仪、超速离心机、移液器等。

关 键 词：Goose paramyxovirus HN protein gene F protein gene CLONING Sequence analysis 

分 类 号：S852.65[农业科学—基础兽医学]