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Cloning and Bioinformatics Analysis of cobQ Gene from Vibrio alginolyticus Strain HY9901
《Asian Agricultural Research》2025年第4期19-23,共5页Chao HUANG Zhihang CHEN Weijie ZHANG Xiaoxin WEN Jiajie MA Peng ZHOU Yingying JIANG Huanying PANG 无 
Natural Science Foundation of Guangdong Province(2025A1515011061);Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University;Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).
[Objectives] To analyze the function of cobQ gene from Vibrio alginolyticus strain HY9901,and to provide a reference for exploring the possible mechanism of cobQ gene from V.alginolyticus.[Methods] A pair of primers w...
关键词：Vibrio alginolyticus Gene cloning cobQ gene Bioinformatics analysis 
Cloning scheme for multipartite entangled pure states via photonic quantum walk
《量子电子学报》2025年第2期206-216,共11页WANG Guocui LIN Zhi LI Xikun YANG Qing YANG Ming 
国家自然科学基金项目(11947102,12004005);安徽省自然科学基金项目(2008085MA16,2008085QA26);安徽省高校协同创新项目(GXXT-2022-039);安徽省高校杰出青年基金项目(2022AH020008)。
The no-cloning theorem has sparked considerable interest in achieving high-fidelity approximate quantum cloning.Most of the previous studies mainly focused on the cloning of single particle states,and cloning schemes ...
关键词：quantum optics entanglement cloning photonic quantum walk multipartite entanglement 
Cloning and Bioinformatics Analysis of CsFK111 Gene from Cucumbers
《Journal of Northeast Agricultural University(English Edition)》2024年第4期16-30,共15页Zhang Hetong Li Li Gao Mei Jia Jincui Xin Ming 
Supported by the National Natural Science Foundation of China(32272724);the National Science Foundation of Heilongjiang Province,China(LH2019C033)。
At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software too...
关键词：F-box gene dwarf cucumber gene cloning bioinformatics analysis 
A PCR-independent,annealing-free cloning method for the insertion of short DNA fragments
《Acta Biochimica et Biophysica Sinica》2024年第12期1886-1890,共5页Linbo Li Jin Yan Yuan Qi Zhenglong Xiang Na Jiang Tongkang Yuan Zhenyi Wang Yuan Wang Huaizhe Zhan Shiyi Liu Li Zhao Jing Xu Xiaowei Lei Yuxuan Liu Gui Wang Jiayang Xie Zhenming Guo Chunhai Cai Shan Bian 
supported by the grants from the National Natural Science Foundation of China(Nos.32271019 to S.B.,and 32300810 to Z.G.);the National Key R&D Program of China(Nos.2020YFA0112500 and 2021YFA1100400 to S.B.);the China National Postdoctoral Program for Innovative Talents(No.BX20220230 to Z.G.);the Shanghai Sailing Program(No.22YF1436100 to Z.G.);the Natural Science Foundation of Shanghai Municipality(No.22ZR1462600 to S.B.);the Fundamental Research Funds for the Central Universities(Nos.22120230454 and 22120230292 to S.B.).
Since two major biotechnological advances,the discovery of restriction endonucleases in the 1970s[1]and the invention of the polymerase chain reaction(PCR)technique in the 1980s[2],molecular cloning has become a funda...
关键词：INSERTION ANNEALING CLONING 
Can imaginarity be broadcast via real operations?
《Communications in Theoretical Physics》2024年第11期66-75,共10页Linshuai Zhang Nan Li 
supported by the National Key R&D Program of China under Grant No.2020YFA0712700;the National Natural Science Foundation of China under Grant No.12341103;the Youth Innovation Promotion Association of CAS under Grant No.2020002。
Imaginarity has proven to be a valuable resource in various quantum information processing tasks.A natural question arises:can the imaginarity of quantum states be broadcast via real operations?In this work,we present...
关键词：imaginarity BROADCASTING CLONING real operations 
Cloning of sft-4 and its influence on vitality and virulence of pine wood nematode,Bursaphelenchus xylophilus
《Journal of Forestry Research》2024年第5期191-200,共10页Shuisong Liu Linsong Wang Ronggui Li Mengyu Chen Wenjun Deng Chao Wang Guicai Du Qunqun Guo 
supported by the Shandong Provincial Natural Science Foundation,China(ZR2020MC123);Qingdao Municipal People-benefitting Demonstration Project of Science and Technology,China(23-2-8-cspz-8-nsh).
In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus g...
关键词：Black pine PINACEAE Bursaphelenchus xylophilus Sft-4 In situ hybridization RNAI PATHOGENICITY 
Cloning of BjNAC102 Promoter and Construction of Expression Vector in Brassica juncea
《Agricultural Science & Technology》2024年第3期14-20,共7页XIAO Xin-bo YUAN Yu-hui YOU Liang HUANG Xin CHEN Ming-zhe MA Yin-hua LIU Xian-jun CHEN Hu 
Supported by Natural Science Foundation of Hunan Province(2023JJ50083,2023JJ50084);Excellent Youth Project of Hunan Provincial Department of Education(22B0844);Hunan Provincial Graduate Research Innovation Project(CX20231274)。
Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea...
关键词：Brassica juncea PROMOTER BjNAC102 Plant expression vector construction 
Cost-Effective Method of Gene Synthesis by Sequencing from Microchip-Derived Oligos for Droplet Cloning
《Advances in Bioscience and Biotechnology》2024年第8期474-485,共12页Kimberly Wang 
Gene synthesis has provided important contributions in various fields including genomics and medicine. Current genes are 7 - 30 cents depending on the assembly and sequencing methods performed. Demand for gene synthes...
关键词：COST-EFFECTIVE Gene Synthesis MICROCHIP Oligo Droplet Cloning 
Cloning, Characterization and Transformation of Methyltransferase 2a Gene (Zmet2a) in Maize (Zea mays L.)
《Phyton-International Journal of Experimental Botany》2024年第7期1767-1779,共13页Xin Qi Yu Wang Xing Zhang Xiaoshuang Wei Xinyang Liu Zhennan Wang Zhenhui Wang Fenglou Ling 
supported by Jilin Province Science and Technology Development Program(20220202014NC);the National Natural Science Foundation of China(#31471565 and#31170259).
DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this stu...
关键词：DNA methylation METHYLTRANSFERASE Zea mays L flowering time functional analysis 
Gene Cloning and Bioinformatics Analysis of phoR Gene from Vibrio alginolyticus HY9901
《Asian Agricultural Research》2024年第6期36-40,共5页Xiangyu LIU Peng ZHOU Haiyun FENG Weijie ZHANG Huanying PANG Na WANG Xiaonan LU 
Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University;National Natural Science Foundation of China(32073015);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802);Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007).
PhoR is a histidine kinase in a two-component regulatory system that regulates phosphorus metabolic pathways and undertakes the key mission of information transmission in pathogenic bacteria.The full-length phoR gene ...
关键词：VIBRIO ALGINOLYTICUS phoR GENE GENE CLONING BIOINFORMATICS analysis 
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