Cloning, Characterization and Transformation of Methyltransferase 2a Gene (Zmet2a) in Maize (Zea mays L.)  

作　　者：Xin Qi Yu Wang Xing Zhang Xiaoshuang Wei Xinyang Liu Zhennan Wang Zhenhui Wang Fenglou Ling 

机构地区：[1]Faculty of Agronomy,Jilin Agricultural University,Changchun,130118,China [2]Rice Research Center,Jilin Agricultural University,Changchun,130118,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第7期1767-1779,共13页国际实验植物学杂志（英文）

基　　金：supported by Jilin Province Science and Technology Development Program(20220202014NC);the National Natural Science Foundation of China(#31471565 and#31170259).

摘　　要：DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this study,a novel methyltransferase 2a gene(Zmet2a)was cloned in maize and identified by polymerase chain reaction-base(PCR-base)using a bioinformatics strategy.The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides.The Zmet2a protein revealed that it contains BAH and CHROMO structural domains,is a non-transmembrane protein that is hydrophilically unstable,and has no signal peptide structure.Meanwhile,we verified the biological roles of Zmet2a using transgenic Arabidopsis overexpressing Zmet2a and Zmet2a-knockout maize.Transgenic Zmet2a Arabidopsis thaliana showed highly significant advancement inflowering time,and Zmet2a-knockout maize showed advancement inflowering time,with significant changes in several traits.Altogether,these report the role of Zmet2a in the regulation offlowering time,which will lay a foundation for revealing the biological function and epigenetic regulation mechanism of Zmet2a in the growth,development andflowering of maize.

关 键 词：DNA methylation METHYLTRANSFERASE Zea mays L flowering time functional analysis 

分 类 号：S513[农业科学—作物学]