Gene Cloning and Bioinformatics Analysis of phoR Gene from Vibrio alginolyticus HY9901  

作　　者：Xiangyu LIU Peng ZHOU Haiyun FENG Weijie ZHANG Huanying PANG Na WANG Xiaonan LU 

机构地区：[1]Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China [2]Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes,Zhanjiang 524025,China [3]Chinese Academy of Inspection and Quarantine,Beijing,100176,China [4]Department of Food Science and Agricultural Chemistry,Faculty of Agricultural and Environmental Sciences,McGill University,Sainte-Anne-de-Bellevue H9X 3V9,Canada

出　　处：《Asian Agricultural Research》2024年第6期36-40,共5页亚洲农业研究（英文）

基　　金：Supported by Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University;National Natural Science Foundation of China(32073015);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802);Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007).

摘　　要：PhoR is a histidine kinase in a two-component regulatory system that regulates phosphorus metabolic pathways and undertakes the key mission of information transmission in pathogenic bacteria.The full-length phoR gene was successfully cloned from the Vibrio alginolyticus HY9901 strain.A comprehensive analysis of the cloned gene was conducted using bioinformatics.Sequence analysis revealed that the total length of the phoR gene(GenBank accession No.:KJ958404.1)is 1299 bp,with the coding region containing a total of 432 amino acid residues.The phylogenetic tree of PhoR revealed that it belongs to the same subclade as V.diabolicus.The SMART program was employed for the purpose of functional domain prediction,which revealed that PhoR possesses three major functional domains:PAS(amino acids 98-166),HisKA(amino acids 205-272),and HATPase_c(amino acids 317-429).

关 键 词：VIBRIO ALGINOLYTICUS phoR GENE GENE CLONING BIOINFORMATICS analysis 

分 类 号：S941.42[农业科学—水产养殖]