Study on the Acute Toxicity and Genetics Toxicity of Bensulfuronk-methyl on Danio rerio  

苄嘧磺隆对斑马鱼的急性毒性和遗传毒性研究

作　　者：姜罡丞[1] 

机构地区：[1]许昌学院,河南许昌461000

出　　处：《Agricultural Science & Technology》2009年第4期128-131,共4页农业科学与技术（英文版）

摘　　要：[Objective] The aim was to study the effect of bensulfuron-methyl herbicide on acute toxicity and genetics toxicity of Danio redo. [ Method] Median lethal concentration was calculated by acute toxicity test, and analyzing the herbicide whether existing in potential toxicity to aquatic organisms or not. Based on the study of acute toxicity, genetics toxicity was carried out, by calculating the micronucleus rate to judge bensulfuron-methyl herbicide whether existing in potential toxicity or not. [ Result ] The LD5o （24 h and 48 h） of bensulfuron-methyl herbicide are 0.698 ml/L and 0.637 ml/L respectively, the safe concentration was 0.159 ml/L. The results on the effects of micronucleus （MN） in erythrocytes of Danio redo induced by bensulfuron-methyl at different times and different concentrations showed that the MN rate of control group was 0.010 3%, the highest MN rate of experimental group reached to 0. 372%, it also indicated that bensulfuron-methyl herbicide had genetics toxicity to Danio redo. At the same detection time, there was dose-effect relationship of MN rate in erythrocytes between treatment and control groups with different concentrations. In the same treatment group, the MN rate in erythrocytes reached to peak value at 24 h, and decreased at 48 h and 72 h with the infection time was prolonged. [ Conclusion ] The study provides some basis for scientifically selecting and reasonably using herbicide.1材料与方法1．1动物供试斑马鱼体重12～17g、体长4—4．5cm，购于宠物市场。实验室驯养7d，每天更换1次暴晒24h的水，选取行动活泼，身体健康的个体进行试验。

关 键 词：Danio rerio BENSULFURON-METHYL MICRONUCLEUS Acute toxicity Genetics toxicity 

分 类 号：X174[环境科学与工程—环境科学]