生长抑素对人肝癌HepG2细胞增殖及cyclinD1、cyclinE蛋白表达的影响  

Effects of somatostatin on proliferation and expression of cyclinD1 and cyclinE in hepatoma cell line HepG2

作　　者：苏秀珍[1] 张辉[1] 梁铁军[1] 

机构地区：[1]山东大学附属省立医院,济南250021

出　　处：《山东医药》2009年第35期16-18,共3页Shandong Medical Journal

摘　　要：目的探讨生长抑素治疗肝癌的作用机制。方法取对数生长期HepG2细胞以不含血清的培养液使其周期同步化，以含血清培养液继续培养24h后随机分为观察Ⅰ、Ⅱ、Ⅲ组及对照组，前三组分别加入质量浓度为100、200、400μg／（kg·d）的生长抑素，对照组加入等体积RPMll640培养液24—72h。MTT比色法观察各组细胞增殖抑制率，流式细胞仪检测细胞周期分布，免疫组化法检测周期素D1（cyclinD1）、周期素E（cyclinE）蛋白表达。结果观察Ⅰ、Ⅱ、Ⅲ组不同时间点细胞增殖抑制率均显著高于对照组，且同一时间点观察Ⅲ组〉观察Ⅱ组〉观察I组，同组中72h〉48h〉24h（P〈0．05、0．01）；观察Ⅰ、Ⅱ、Ⅲ组G，期细胞比例均显著高于对照组，观察Ⅱ、Ⅲ组S期细胞比例均显著低于对照组（P〈0．05、0．01）；观察Ⅰ、Ⅱ、Ⅲ组cyclinD1、cyclinE蛋白水平均显著低于对照组，且观察Ⅲ组〈观察Ⅱ组〈观察Ⅰ组（P〈0．05、0．01）。结论生长抑素可通过下调cyclinD1、cyclinE表达抑制HepG2细胞增殖，此可能为其治疗肝癌的作用机制之一。Objective To investigate the mechanism of somatostatin for treating hepatoma. Methods HepG2 cells in logarithmic growth phase were obtained and cultured to be celt cycle synchronization with serum-free medium, then cultured with medium for 24 hs and divided randomly to observed groups Ⅰ , Ⅱ, Ⅲ and control group, the former three groups were treated with somatostatin at concentrations of 100,200 and 400 μg/（ kg·d） respectively,and the control group treated with RPMI1640 culture medium for 24-72hs. The cell proliferation inhibition rate（CPIR） in all groups was detected by MTT assay ,cell cycle distribution was observed by flow eytometr,eyclin D1 and eyclin E protein expression were detected by immunohistochemistry assay. Results CPIR in observed groups Ⅰ , Ⅱ, Ⅲ at different times were significantly higher than those in the control group, and CPIR in observed group Ⅲ 〉 group Ⅱ 〉 group Ⅰ at the same time, CPIR at 72 h 〉 48 h 〉 24 h in the same group（P 〈 0.05,0.01 ）. The percentage of G1 phase cells in observed groups Ⅰ , Ⅱ, Ⅲ were significantly higher than that in the control group, the percentage of S phase cells in observed groups Ⅱ and Ⅲ were significantly lower than that in the control group（ P 〈 0.05,0.01 ）. cyclin D1 and cyclin E protein level in observed groups Ⅰ , Ⅱ , Ⅲ were significantly lower than that in the control group, and which in observed group Ⅲ〉 group Ⅱ 〉 group Ⅰ at the same time （ P 〈 0.05, 0.01 ）. Conduslons Somatostatin can inhibit the proliferation of hepatoma cell line HepG2 by down-regulating the expression of cyclin D1 and cyclin E,which maybe its mechanism for treating hepatoma.

关 键 词：生长抑素 人肝癌HEPG2细胞 细胞增殖 周期素 

分 类 号：R735.7[医药卫生—肿瘤]