Molecular Characterization and SNP Markers of the β-purothionin Gene in Einkorn Wheats  

作　　者：LI Jing-qiong ZHENG You-liang WEI Yu-ming 

机构地区：[1]Triticeae Research Institute, Sichuan Agricultural University, Ya ＂an 625014, P.R. China [2]Key Laboratory of Crop Genetic Resources and Improvement in Southwest China, Ministry of Education/Sichuan Agricultural University, Ya＇an 625014, P.R.China

出　　处：《Agricultural Sciences in China》2009年第9期1019-1027,共9页中国农业科学（英文版）

基　　金：supported by the National Key Tech-nologies R&D Program of China (2006BAD01A02-23and 2006 BAD13B02-06);the National High Tech-nology Research and Development Program of China(863 Program, 2006AA10Z179 and 2006AA10Z1F8)

摘　　要：Forty-three gene sequences encoding purothionin were characterized from the three species or subspecies of einkorn wheats. These sequences contained 887 bp, among which 92 SNPs including 29 indel loci were detected, giving an average SNP frequency of one SNP per 9.64 bases. According to these sequences, 5 SNP markers were successfully designed, which were used to mine the variations ofpurothionin genes of 102 einkorn wheat accessions. Based on the 5 detected SNP loci, 102 einkorn wheat accessions could be divided into 21 haplotypes, among which 11 haplotypes contained a single sample. Phylogenetic analysis indicated that the purothionin genes from einkorn wheats were more closely related to those from D genome than B genome. Seven out of the 43 gene sequences were assumed to be pseudogenes by the definition of containing in-frame stop codons and small insertions/deletions leading to frameshift. In the remaining 36 amino acid sequences, the 8 Cys and Tyr-13 loci in the mature thionin domain which played important roles in the biological activities were all conserved, whereas there were some varieties occurred in some other important amino acid residues such as Lys and Arg.Forty-three gene sequences encoding purothionin were characterized from the three species or subspecies of einkorn wheats. These sequences contained 887 bp, among which 92 SNPs including 29 indel loci were detected, giving an average SNP frequency of one SNP per 9.64 bases. According to these sequences, 5 SNP markers were successfully designed, which were used to mine the variations ofpurothionin genes of 102 einkorn wheat accessions. Based on the 5 detected SNP loci, 102 einkorn wheat accessions could be divided into 21 haplotypes, among which 11 haplotypes contained a single sample. Phylogenetic analysis indicated that the purothionin genes from einkorn wheats were more closely related to those from D genome than B genome. Seven out of the 43 gene sequences were assumed to be pseudogenes by the definition of containing in-frame stop codons and small insertions/deletions leading to frameshift. In the remaining 36 amino acid sequences, the 8 Cys and Tyr-13 loci in the mature thionin domain which played important roles in the biological activities were all conserved, whereas there were some varieties occurred in some other important amino acid residues such as Lys and Arg.

关 键 词：einkorn wheats purothionin SNP HAPLOTYPE 

分 类 号：S512.1[农业科学—作物学]