一株高产蛋白酶芽孢杆菌的鉴定  

Identification of high-yield protein producing Bacillus strain

作　　者：刘海军[1] 乐超银[1] 邵伟[2] 王健[1] 李金鞠[1] 梁薇[1] 

机构地区：[1]三峡大学生物技术中心,湖北宜昌443002 [2]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《中国酿造》2009年第9期18-20,共3页China Brewing

基　　金：中国博士后基金项目资助(20060390775)

摘　　要：通过经典生理生化实验和16S rRNA序列分析,对新分离出的1株高产蛋白酶的芽孢杆菌进行系统鉴定。研究发现该菌卵磷脂酶试验和马尿酸盐反应均为阳性,并在60℃仍有蛋白酶活性。将该菌16S rRNA序列在Genbank中进行blast,发现其与枯草芽孢杆菌EHFS1-S02Hc的16S rRNA序列同源性高达99.63%,分离出的高产蛋白酶芽孢杆菌可能为一新亚种或生物型。An isolated Bacillus strain with higher- yield protease was identified systematically through the physiological and biochemical detection method and the 16S rRNA sequence analysis. The results showed that the Bacillus strain had higher hydrolytic ability and showed positive lecithinase, hippurate. The protease activity was still available under 60℃. The homology of 16S rRNA sequence of this strain was up to 99.63% compared with Bacillus subtilis EHFS1 - S02Hc. Therefore, the identified Bacillus strain might be a new subspecies or a biovariety of the species.

关 键 词：菌种鉴定 芽孢杆菌 16SrRNA 蛋白酶 水解特性 

分 类 号：Q93-331[生物学—微生物学]