重组人aFGF工程菌发酵条件的优化  

Optimization of Condition for Fermentation of Recombinant E. coli with Human aFGF Gene

作　　者：王树民[1] 解佳森[1] 刘丹[2] 杜金霞[1] 王晓杰[1] 田海山[1] 李校堃[1] 

机构地区：[1]吉林农业大学生物反应器与药物开发教育部工程研究中心,长春130118 [2]长春生物制品研究所细胞因子室,长春130062

出　　处：《中国生物制品学杂志》2009年第9期898-900,共3页Chinese Journal of Biologicals

基　　金：长春净月科技项目(200613001)

摘　　要：目的优化重组人酸性成纤维细胞生长因子(rhaFGF)工程菌的发酵条件。方法采用分批补料方式,在菌体快速生长阶段,通过变速流加碳源和氮源,控制流加比例,调节pH值和溶解氧(DO)含量,实现工程菌的高密度发酵及目的蛋白的高效表达。结果当碳源与氮源流加比例为3:1时,菌体湿重可达145g/L,干重达36g/L,目的蛋白表达量达28.9%;DO控制在20%时,全菌体和裂解液中目的蛋白的表达量均最高,分别可达29.11%和44.28%。结论已初步优化了rhaFGF工程菌的发酵条件。Objective To optimize the condition for fermentation of recombinant E. coli with human acidic fibroblast growth factor （aFGF） gene. Methods The recombinant E. eoli with human aFGF gene was fermented by fed batch. The pH value and DO at rapid growth stage of bacteria were regulated by supplement of carbon and nitrogen sources at variable flowing rate and ratio. Resuits When the ratio of carbon source to nitrogen source was 3 ： 1, the wet and dry weights of bacteria reached 145 and 36 g/L respectively, and the expression level of target protein was 28. 9%. When the DO was controlled at 20%, both the expression levels of target protein in the whole bacterial cells and bacterial lysate reached the peak values which were 29. 11% and 44. 28% respectively. Conclusion The condition for fermentation of recombinant E. coli with human aFGF gene was preliminarily optimized.

关 键 词：人酸性成纤维细胞生长因子 工程菌 发酵 溶氧量 

分 类 号：R986[医药卫生—药品] R382.31[医药卫生—药学]