变形链球菌表面蛋白DNA防龋疫苗的构建  

Construction of a S. mutqns surface protein DNA vaccine against dental caries

作　　者：樊明文[1] 陈罕[1] 边专[1] 张平[1] 

机构地区：[1]湖北医科大学口腔医学院,武汉市4730070

出　　处：《口腔医学纵横》1998年第3期131-133,共3页Journal of Comprehensive Stomatology

基　　金：国家自然科学基金!39770799

摘　　要：目的旨在构建一种新型的表面蛋白防龋疫苗—DNA防龋疫苗。方法从表面蛋白pac基因上利用BamHI和Sacl双酶切，获得一个3281bp的片段。将该片段定向插入真核表达载体PSVL，再将连接后新构成的质粒pSVL／pac转入氯化钙法制备的感受态大肠杆菌E．coliDH5a。用BamHI和SacI双酶切，琼脂糖电泳证实pSVL／pac构建的正确性。结果用定向克隆的方法获得了一个含pac基因的真核表达质粒pSVL／pac即表面蛋白DNA疫苗。结论利用定向克隆和氯化钙法可以较为简便和快速地构建表面蛋白的DNA防龋疫苗。Objective To construct a new type anticaries vaccine that was S. mutans surface protein DNA vaccine.Method The 328fop BamHI - Sacl fragment containing truncated pac gene from S. mutans MT8148 was directedly inserted into eukaryotic expression vector PSVL to create pSVL/pac. For amplifying plasmid pSVL/pac. plasmid pSVL/pacwas transformed into E. colt DH5y. Plasmid pSVL/pac was digested by BamHI.Sacl and BamHI - SacI, then run 1 % agarose gel electrophoresis to confirm the correction of pSVL/pac construction. Result A S. mutans surface protein DNAvaccine was constructed by direct coloning to create a eukaryotic expression system pSVL/pac. Conclusion A S. mutans surface protein DNA vaccine could be constructed by direct coloning.[

关 键 词：变形链球菌 DNA疫苗 表面蛋白 龋齿 预防 

分 类 号：R780.2[医药卫生—口腔医学] R780.1[医药卫生—临床医学]