重组人IL-6在沙门菌x4550中的表达  

Expression of recombinatant human interleukin 6 in salmomella typhimurium x4550

作　　者：赵国强[1] 苏堤[1] 程勉学[1] 阮丽荣[1] 龚光明[1] 贺向荣[1] 王东升[1] 

机构地区：[1]河南医科大学微生物学与免疫学教研室

出　　处：《河南医科大学学报》1998年第3期89-91,共3页Journal of Henan Medical University

摘　　要：为在鼠伤寒沙门菌ｘ４５５０中表达重组人白细胞介素６（ＩＬ６），利用定点突变、聚合酶链反应、体外重组ＤＮＡ技术构建出重组表达载体ｐＹＡ３３３３ＩＬ６；利用ＩＬ６依赖性的小鼠杂交瘤细胞株Ｂ９测定重组人ＩＬ６ＨＧＦ的活性。结果：从全菌ＳＤＳＰＡＧＥ考马斯亮蓝染色后薄层扫描分析表达产物产率为１７６％，相对分子质量为２１×１０４；测定重组人ＩＬ６ＨＧＦ活性为２×１０６Ｕ／Ｌ。提示：鼠伤寒沙门菌ｘ４５５０能够表达人ＩＬ６。In order to express recombinant human interleukin 6 (rhIL 6) in Salmonella typhimurium x4550,a recombinant expression plasmid pYA3333 IL6 was constructed by using PCR amplification,site specific mutagenesis and DNA recombination in vitro.Hybridoma growth factor (HGF) activity of rhIL 6 was measured with an IL 6 dependent B cell hybridoma line B9.Results showed that the expressed rhIL 6 relative molecular weight 2 1×10 4 was accounted for 17.6% of the S.typhimurium proteins and the biological activity of the expressed rhIL 6 was 2×10 6 U/L bacterial medium,suggesting that salmonella typhimurium x4550 could be used to express recombinant human interleukin 6.

关 键 词：白细胞介素6 重组 伤寒沙门氏菌 

分 类 号：R378.23[医药卫生—病原生物学] R392.12[医药卫生—基础医学]