三种水生植物内生真菌多样性及其抗真菌活性  

Diversity of Endophytic Fungi from Three Aquatic Plants and Their Antagonistic Activities in vitro

作　　者：赵春安[1] 罗珊珊[1] 毕艳艳[1] 李海燕[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650224

出　　处：《微生物学通报》2009年第9期1305-1310,共6页Microbiology China

基　　金：昆明理工大学人才培养基金项目(No.KKE2007038)

摘　　要：从华凤仙(Impatiens chinensis L.)、问荆(Equisetum arvense L.)和轮叶狐尾藻(Myriophyllum verticillatum L.)3种植物中共分离到内生真菌155株。经鉴定,它们分属于26个不同的分类单元。以杨桃炭疽菌(Colletotrichum gloeosporioides)、香蕉疫霉(Phytophthora nicotianae)和腐皮镰刀菌(Fusarium solani)等6种植物病原真菌为指示菌,对3种水生植物内生真菌代谢产物进行抗菌活性研究。结果表明,有37株内生真菌(占分离菌株总数的23.9%)显示出对一种或多种病原菌的抑菌活性。来自华凤仙、问荆和轮叶狐尾藻的抗菌活性菌株比例分别为29.2%、13.9%及37.1%,都远低于已报道的陆生植物内生真菌中活性菌株的比例。抗菌活性菌株主要分布于枝孢属(Cladosporium)、木霉属(Trichoderma)和地霉属(Geotrichum)等5个属中。155 endophytic fungi were isolated from Equisetum arvense L., Impatiens chinensis L. and Myriophyllum verticillatum L. They were identified to 26 taxa. The antagonistic activities of these isolates against 6 isolates of plant pathogenic fungi were tested on the medium, and 37（23.9%） isolates were activitive against one or more phytopathogens. The percentage of antifungal active isolates from E. arvense L., L chinensis L. and M. verticillatum L. were 13.9%, 29.2% and 37.1%, respectively. It was lower than that of terrestrial plants. The active isolates were mainly belonging to 5 genera including Cladosporium, Trichoderma and Geotrichum.

关 键 词：水生植物 内生真菌 枝孢属 抑菌活性 

分 类 号：Q93[生物学—微生物学]