质子自杀法选育克雷伯氏菌产乳酸突变株  

Isolation and Characterization of Klebsiella oxytoca M5al Mutants with Proton Suicide Method

作　　者：王红兵[1] 管桂萍[2] 田杰生[3] 

机构地区：[1]湖南省畜牧兽医研究所,湖南长沙410131 [2]湖南农业大学生物工程系,湖南长沙410128 [3]中国农业大学微生物与免疫学系,北京10094

出　　处：《微生物学通报》2009年第9期1368-1371,共4页Microbiology China

摘　　要：以产酸克雷伯氏菌(Klebsiella oxytoca)M5al为出发菌株,经亚硝基胍诱变处理,运用质子自杀法选育,从含0.17mol/L NaBr-NaBrO3的初筛平板上选出44个具有稳定遗传性的单菌落,然后结合培养基优化后的摇瓶发酵复筛,获得3个产乳酸突变株,其乳酸脱氢酶活性分别为出发菌株的50.6%、58.8%、61.3%。对其中乳酸脱氢酶活性最低的菌株在5L自动发酵罐上进行批式发酵,结果显示:突变株乳酸产量大幅降低,而乙酸、1,3-丙二醇的产量则显著增加。Klebsiella oxytoca M5al was used as the original strain to breed mutants after mutagenesis with NTG 44 single colony were obtained from medium containing 0.17 mol/L NaBr-NaBrO3 （proton suicide method） and 3 lactic acid-producing mutants were selected by shaking cultivation in optimized medium, their lactate dehydrogenase activity was only 50.6%, 58.8%, 61.3% of the parent strain, respectively. The lowest one of them batch-cultured in 5 L fermenter, results indicated that the yield of lactic acid of the mutant was decreased remarkably, reversely, acetic acid and 1,3-propanediol were increased observably.

关 键 词：质子自杀 产酸克雷伯氏菌 产乳酸突变株 乳酸脱氢酶 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]