小麦抗叶锈近等基因系TcLr38 RGAs分析  

RGA Analysis of Near-isogenic Line TcLr38 Resistance to Wheat Leaf Rust

作　　者：王文霞[1] 宋志强[1] 杨文香[1] 刘大群[1] 

机构地区：[1]河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《中国农学通报》2009年第19期222-227,共6页Chinese Agricultural Science Bulletin

基　　金：河北省自然科学基金项目"小麦抗叶锈基因Lr19表达序列标签的研究"(C2006000438);高校博士点基金项目"小麦近等基因系TcLr38;TcLr45特定染色体文库的构建"(20050086002);国家十一五支撑计划"小麦重大病虫害防控技术小麦叶锈病毒性基因检测与抗叶锈性鉴定"(2006BAD08A05)

摘　　要：为获得Lr38的抗病类似物质,寻找与抗病基因表达相关的目的片段。根据已知植物抗病基因的NBS-LRR(核苷酸结合位点-富含亮氨酸重复)类保守区域设计简并引物,应用RGA技术在小麦的未知基因cDNA中扩增和分离抗病基因同源序列。从小麦抗叶锈近等基因系TcLr38中获得了9个小麦抗病基因同源片段D-8、A-5、B-20、C-6、F-16、A-4、B-9、C-4和D-12。经BLASTp分析,9个片段都含有NB-ARC保守结构域,其中D-8、A-5、B-20、C-6、A-4、B-9、C-4和D-12与已知抗病基因的相应区域相一致,具有抗病基因NBS特征结构域激酶2a(Kinase-2a)、激酶3a(Kinase-3a)和疏水结构域(HD)。片段D-8、A-5、B-20、C-6、A-4、B-9、C-4和D-12可能与抗病基因表达相关。The aim of the research is to isolate the Resistance gene analogs（RGAs） of Lr38, further to detect the fragments that relat to disease-resistant gene expression. Degenerate primers designed according to conserved NBS-LRR （nucleotide binding site-leucine rich repeat） regions of known disease resistance（R） genes was used to amplify and clone homologous sequences from wheat leaf rust near-isogenic TcLr38 by RGAs （Resistance gene analogs）. Nine resistance gene analogs D-8, A-5, B-20, C-6, F-16, A-4, B-9, C-4 and D-12 were obtained from wheat leaf rust near-isogenic TcLr38. Nine isolated RGAs contained the conserved motifs of NB-ARC after the BLASTp analysis, and D-8, A-5, B-20, C-6, A-4, B-9, C-4 and D-12 were deduced corresponding to NBS-containing genes, such as Kinase-2a, kianse-3a and hydrophobic domain（HD）. D-8, A-5, B-20, C-6, A-4, B-9, C-4 and D-12 were supposed to disease-resistant gene expression.

关 键 词：小麦叶锈 TcLr38 NBS—LRR 抗病基因同源序列 

分 类 号：S432.1[农业科学—植物病理学]