北方杂交粳稻亲本遗传背景分析  

Genetic Basis of Parents of japonica Hybrid Rice in the Northern China

作　　者：王昌华[1,2] 张燕之[2] 华泽田[2] 徐正进[1] 郑文静[2] 赵家铭[2] 刘欣[3] 王辉[2] 周毓珩[2] 

机构地区：[1]沈阳农业大学稻作研究所,辽宁沈阳110161 [2]辽宁省农业科学院稻作研究所,辽宁沈阳110161 [3]沈阳农业大学生物技术学院,辽宁沈阳110161

出　　处：《中国水稻科学》2009年第5期489-494,共6页Chinese Journal of Rice Science

基　　金：农业部农业科技跨越计划资助项目(2007GB000060);辽宁省稻作研究所青年人才启动专项基金资助项目

摘　　要：为了揭示北方杂交粳稻亲本的遗传背景,利用微卫星(SSR)分子标记对北方杂交粳稻亲本及其高代后续材料进行了遗传差异鉴定和籼性程度分析。26对SSR引物共扩增出71个等位基因,每对引物平均等位基因数为2.73个。用非加权类平均法(UPGMA)进行聚类分析,供试材料在相似系数0.66处可分成5组,各组间材料的遗传差异相对较大;64个恢复系材料的籼性程度参数值(ADi)的变幅为0.056～0.684;24个不育系材料ADi的变幅为0.087～0.381。分析表明,强优势杂交粳稻组合双亲之间遗传距离应在0.30～0.60,恢复系的ADi应在0.26～0.50,不育系的ADi不应高于0.20,杂交组合的ADi应在0.20～0.40,同时杂交粳稻组合优势受母本的遗传背景的影响较大,母本自身应具有较高的产量潜力。To reveal the genetic basis of parents of japonica hybrid rice in the Northern China, genetic differentiation and indiea component analysis were conducted on the main parents of japonica hybrid rice and their succeeding materials by using simple sequence repeats（SSR） marker. The genomic DNAs of 96 rice materials were amplified with 26 primers, and a total of 71 alleles were detected, with 2.73 alleles per primer on average. The molecular phylogenetie tree built by UPMGA method showed that the rice materials were divided into 5 groups at the genetic similarity coefficient of 0.66. Relatively great genetic differentiation was found among groups. The extent of indica component value （ADi） of the 64 restorer lines was from 0. 056 to 0. 684, and that of 24 sterile lines was from 0. 087 to 0. 381. It is suggested that the extent of indica component value （ADi） of a hybrid rice combination with high heterosis should be from 0.20 to 0.40, that of its restorer line should be from 0.26 to 0.50, and that of its sterile line should he below 0.20, and the genetic distance of its parents should vary from 0.30 to 0.60. Besides, the female parent of a hybrid rice combination with high heterosis should have a high yield potential, because the yield of a hybrid rice combination was more affected by its sterile line than its restorer line.

关 键 词：杂交水稻 不育系 恢复系 微卫星标记 遗传差异 籼性程度 

分 类 号：S511.22[农业科学—作物学]