SerpinH1对小鼠肿瘤细胞增殖的影响  

Effect of SerpinH1 on Proliferation and Growth of Murine Tumor

作　　者：俞银燕[1] 曾彬[1] 柴立民[1] 杨曙光[1] 李长林[1] 张园[1] 杨荣存[1] 

机构地区：[1]南开大学医学院免疫学教研室,天津300071

出　　处：《肿瘤防治研究》2009年第9期741-744,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30771967);国家科技部基金资助项目(06C26211200695);国家高技术研究发展计划(863计划)资助项目(2006AA020502);天津市科委基金资助项目(07JCZDJC03300;06ZHCXSH04800;08JCYBJC07100)

摘　　要：目的研究SerpinH1基因对肿瘤细胞增殖的影响。方法克隆SerpinH1基因,建立相关稳定转染细胞系,分为5组,分别为空白对照组,pcDNA3.1/V5-SerpinH1实验组,pcDNA3.1/V5对照组,pNKRY-SerpinH1-siRNA实验组,pNKRY-GFP对照组。应用BrdU渗入法,流式细胞术检测,WST-1方法分别测定细胞增殖情况,并建立肿瘤细胞模型,用体内试验验证SerpinH1对肿瘤细胞生长情况的影响。结果过表达基因的pcDNA3.1/V5-SerpinH1组肿瘤生长有抑制作用,而用siRNA干扰技术沉默基因表达的pNKRY-SerpinH1-siRNA组肿瘤生长有促进作用。结论SerpinH1基因能够抑制肿瘤细胞生长,提示SerpinH1具有抑制肿瘤的功能。Objective To investigate the effects of SerpinH1 on the proliferation of murine tumor. Meth- ods We first cloned SerpinH1 and set up SerpinH1 targeted siRNA structure, and then investigated the effects of SerpinH1 on the tumor cell proliferation and growth. The inhibition of cell proliferation and growth was demonstrated by both WST-1 cell proliferation assay and 5Cbromo-2Cdeoxy-uridine in- corporation assay with flow cytometry （FACS）. Results The expression of SerpinH1 could inhibit the proliferation of the monocyte tumor RAW264. 7 cells and melanoma B16 cells. Meanwhile silencing SerpinH1 could promote the proliferation of both RAW264. 7 and B16 cells. Conclusion The results indicated that SerpinH1 could inhibit the proliferation and growth of both RAW264. 7 and B16 tumor cells.

关 键 词：SerpinH1 增殖 肿瘤细胞 

分 类 号：R73-354[医药卫生—肿瘤]