莪术醇对人宫颈癌CASKI细胞增殖抑制及促凋亡作用的研究  

Effects of curcumol on proliferation and apoptosis in human cervical cancer CASKI cell line

作　　者：高艳娥[1] 郭金珠[1,2] 惠慧[1] 王咏雪[1] 王桂贤[1] 

机构地区：[1]西安交通大学医学院第二附属医院妇产科,陕西西安710004 [2]西电集团医院妇产科,陕西西安710077

出　　处：《现代肿瘤医学》2009年第10期1836-1839,共4页Journal of Modern Oncology

基　　金：陕西省中医管理局科技项目(No.2005029)

摘　　要：目的:探讨莪术醇对人宫颈癌CASKI细胞体外增殖和凋亡的影响。方法:12.5μg/ml、25μg/ml、50μg/ml和100μg/ml不同浓度莪术醇作用于人宫颈癌CASKI细胞后,MTT法检测CASKI细胞的增殖抑制率;流式细胞仪分析细胞周期分布及细胞凋亡率变化;电镜观察肿瘤细胞凋亡的形态学特征。结果:12.5μg/ml、25μg/ml、50μg/ml和100μg/ml的莪术醇对宫颈癌CASKI细胞均有不同程度的增殖抑制作用,在一定范围内,具有浓度-时间依赖性。流式细胞仪分析结果显示:50μg/ml、100μg/ml莪术醇作用于宫颈癌CASKI细胞24h,可阻滞细胞周期于G2/M期,并诱导部分细胞凋亡。电镜下可见凋亡细胞及凋亡小体,且100μg/ml组细胞凋亡改变较50μg/ml组更明显。结论:莪术醇能明显抑制CASKI细胞的体外增殖,且可阻滞CASKI细胞周期于G2/M期并诱导细胞凋亡。Objective：To investigate the effects of curcumol on proliferation and apoptosis in human cervical cancer CASKI cell line. Methods： CASKI cells cultured in vitro were separately treated with curcumol at concentration of 12.5,25,50 and 100μg/ml. The cell proliferation was evaluated by Methyl thiazolyl tetrazolium （MTT） assay. Cell cycle distribution and apoptosis were detected by flow cytometry （FCM）. Cell morphology was studied by transmission electron microscope. Results： When CASKI cells were treated with 12.5,25,50 and 100μg/ml curcumol, the prolif- eration of CASKI cells was apparently inhibited in a dose - and time - dependent manner. When CASKI cells were treated with curcumol at 50 and 100μg/ml for 24 h, the CASKI cells were arrested in G2/M phase and showed typical apoptosis characteristics in FCM and transmission electron microscope analysis. Conclusion ： Curcumol can inhibit the proliferation of CASKI cells,block cell cycle at the G2/M phase,and induce cell apoptosis.

关 键 词：莪术醇 宫颈癌 CASKI细胞 细胞周期 凋亡 

分 类 号：R730.55[医药卫生—肿瘤] R737.33[医药卫生—临床医学]