肝癌相关肿瘤抗原AFP新的HLA-A2/A24限制性CTL表位的预测及分析  

Prediction and analysis of a novel HLA-A2/A24-restricted CTL epitope derived from AFP antigen associated with hepatocellular carcinoma

作　　者：张秀敏[1] 王军伟[1] 李增山[1] 郭风[2] 隋延仿[1] 

机构地区：[1]第四军医大学基础部病理学教研室,陕西西安710032 [2]第四军医大学西京医院消化科,陕西西安710032

出　　处：《现代肿瘤医学》2009年第10期1839-1842,共4页Journal of Modern Oncology

基　　金：国家高技术研究发展计划项目("863"计划)(编号:2007AA02Z470);陕西省攻关项目[编号:2007K09-05(2)]

摘　　要：目的:预测肝癌肿瘤抗原AFP新的HLA-A2/A24限制性CTL表位,为肝癌CTL表位肽的筛选及鉴定提供依据。方法:选择与肝癌密切相关的肿瘤抗原AFP为研究目标,采用NetCTL1.2Server远程预测系统进行HLA-A2/A24限制性CTL表位预测。结果:共预测出与肝癌相关的肿瘤相关抗原AFP的8个HLA-A2限制性CTL表位,13个HLA-A24限制性CTL表位。结论:应用NetCTL1.2Server法预测CTL表位是一种高效准确的预测方法。所预测的肿瘤抗原AFP的HLA-A2/24限制性CTL表位经实验筛选、鉴定后,可为肝癌治疗性疫苗的制备选择合适的CTL表位提供依据。Objective ： To predict the HLA - A2/A24 restricted CTL epitopes of antigen AFP, associated with hepatocellular carcinoma （HCC）, so as to provide appropriate CTL epitope for hepatocellular carcinoma. Methods： HLA - A/A24 - restricted CTL epitopes of antigen AFP were predicted with NetCTL1.2 Server on web, COMB 〉 1.0. Results： Eight HLA - A2 and thirteen HLA - A24 restricted CTL epitope candidates （nonamers） derived from the tumor antigen AFP associated with HCC were predicted. Conclusion： NetCTL 1.2 Server prediction method improve the prediction efficiency and accuracy. These nonamers, after identified by experiment will be useful to develop promising antigen peptide tumor vaccines for HCC.

关 键 词：甲胎蛋白 HLA—A2/A24抗原 细胞毒性T淋巴细胞表位 预测 

分 类 号：R735.7[医药卫生—肿瘤] R73-362[医药卫生—临床医学]