拓扑替康对食管癌细胞株Eca-109的放射增敏作用及机制  

Effects of topotecan on radiosensitization of esophageal carcinoma cell line Eca-109 and its mechanism

作　　者：张晓智[1] 李洁[1] 巩会杰[1] 侯磊[1] 陈葳[2] 

机构地区：[1]西安交通大学医学院第一附属医院放疗科,陕西西安710061 [2]西安交通大学医学院第一附属医院实验医学中心,陕西西安710061

出　　处：《现代肿瘤医学》2009年第10期1850-1853,共4页Journal of Modern Oncology

摘　　要：目的:研究拓扑替康(topotecan,TPT)对人食管癌细胞株Eca-109的放射增敏作用及机制。方法:MTT法检测TPT对Eca-109细胞增殖抑制作用,克隆形成实验检测TPT对Eca-109的放射增敏效应;单击多靶模型拟合细胞存活曲线并计算放射增敏比。相差显微镜观察肿瘤细胞形态学改变,流式细胞仪检测细胞周期及凋亡率。结果:TPT对Eca-109细胞有增殖抑制作用,且呈时间-剂量依赖性,10、20、40mg/LTPT的放射增敏比分别为1.22、1.29、1.36;增敏组凋亡率、G2/M期细胞比例较对照组增多(P<0.05)。结论:TPT对食管癌细胞系Eca-109有放射增敏作用,其机制可能与促进细胞凋亡及引起G2/M期阻滞有关。Objective：To investigate the radiosensitization of topotecan （TPT） on esophageal cancer cell line Eca - 109 and its possible mechanism. Methods：MTT assay was used to measure the effect of TPT on Eca - 109 cell proliferation. Clonogenic assay was performed to determine the radiosensitizing effect of TPT on Eca - 109 cell line. Single - hit muhitarget mode was used to plot survival curve. The morphological changes of Eca - 109 were observed by phase contrast microscope. The cell cycle and apoptosis were measured by flow cytometry. Results： TPT in a time - dose- dependent manner inhibited Eca -109 cell line proliferation, the radiosensitization enhancement ratios （SER DO） of 10,20 and 40mg/L TPT were 1.22,1.29 and 1.36 respectively. The radiosensitization group had a higher apoptosis rate and G2/M phase cells （P 〈 0.05 ）. Conclusion： TPT could enhance the radiosentivity of human esophageal cancer cell line Eca- 109, which possibly associated with encouraging apoptosis and blocking Eca - 109 in G2/M phase.

关 键 词：食管癌 拓扑替康 放射增敏 

分 类 号：R73-36[医药卫生—肿瘤] R735.1[医药卫生—临床医学]