槟榔叶片总蛋白质提取及双向电泳条件初探  

Establishment of Methods for the Extraction and Two-Dimensional Electrophoresis Analysis of Leaf Proteins of Areca catechu

作　　者：李涛[1] 曾莉娟[1,2] 王健华[1] 刘志昕[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海口571101 [2]中国热带农业科学院科技信息研究所,海南儋州5737171

出　　处：《热带作物学报》2009年第6期746-750,共5页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划课题"热带农林重要有害生物检测监测预警技术研究"(No.2007BAD48B01)资助

摘　　要：采用3种提取植物组织蛋白的方法(TCA/丙酮沉淀法、E-TCA法、酚法)提取槟榔叶片总蛋白,在蛋白产量,一维和二维电泳图谱等方面对3种方法的提取效果进行比较。结果表明,TCA/丙酮沉淀法效果较好,是槟榔叶片总蛋白提取的可选方法,同时优化了双向电泳的条件和上样量。Proteins from betel nut （A reca catechu Linn.）were extracted by using three methods [trichloroacetic acid （TCA）/acetone precipitation, trichloroacetic acid precipitation （E-TCA）, and phenol extraction], and their productivity and profiles were assessed by means of SDS-PAGE and two-dimensional electrophoresis. Of these 3 methods the TCA/acetone precipitation method was found most effective for the extraction and two-dimensional electrophoresis of proteins from A reca leaves. The conditions of two- dimensional electrophoresis and protein loading volume were optimized.

关 键 词：槟榔 蛋白提取 二维电泳 

分 类 号：S567.9[农业科学—中草药栽培]