树突状细胞在体外诱导FBL-3细胞分化为单核细胞的研究  

Study of the mechanism of dendritic cells induce FBL-3 cells to differentiate into the monocytes in vitro

作　　者：叶欣[1] 于连玲[2] 赵梅[1] 宋文刚[2] 周宝琴[2] 杨雪[1] 徐兴鲁[1] 

机构地区：[1]泰安市中心医院肿瘤中心 271000 [2]泰山医学院

出　　处：《国际肿瘤学杂志》2009年第9期712-716,共5页Journal of International Oncology

摘　　要：目的探讨树突状细胞（DC）对白血病细胞的诱导分化作用机制。方法应用不同浓度的DC培养上清作用于小鼠红白血病细胞（FBL-3细胞），作用72h后用瑞氏染色观察分化后细胞的大体形态，透射电镜观察分化后细胞的超微结构，流式细胞术观察分化后细胞表面分子CD14的表达情况。结果瑞氏染色、透射电镜、流式细胞术结果均显示，DC培养上清能诱导白血病细胞分化为单核细胞，其作用强度与DC培养上清中IL-12浓度呈正相关。结论DC培养上清可诱导FBL-3细胞分化成为单核样细胞，分化后的细胞在大体形态、超微结构、细胞表型分子标志方面符合单核细胞的特点。且DC培养上清诱导FBL-3细胞分化成为单核样细胞的能力与培养上清中IL-12的浓度呈正相关。Objective To investigate mechanism involved in the differentiation of FBL-3 cells （erythroleukemia cells）induced by dendritic cells（DC）. Methods To cultural the FBL-3 cells together with the DC supernatant of different concentration for 72 hours,then we used the Wright~ staining methed to record the mature monocyte cell population, the transmission electron microscope to observe the uhramicrostructure, the flow cytometry to detect the expression rate of the surface molecular CDI4. Results The Wrights staining methed, the transmission electron microscope and the flow cytometry all presented that after being induced by the supernatant of the DC, the FBL-3 cells can partly differentiate into monocytes, inversion monocyte consistent with the personal characteristics. And the intensity of the DC supernatant was connected with interleukin-12. Conclusion The DC supernatant can induce the FBL-3 cells into monocytoid cell, the differentiated cells correspond to monocyte in macroappearance, uhramicrostructure and phenotype. The competence of the DC supernatant are partly concerned with interleukin-12.

关 键 词：树突细胞 白血病 白细胞介素-12 单核细胞 

分 类 号：R73-3[医药卫生—肿瘤] R329.2[医药卫生—临床医学]