Liquid Chromatography-Electrospray Ionization Mass Spectrometry Method for Determination of Protopanaxadiol in Rat Plasma  

作　　者：WU Yi TENG Guo-sheng LIANG Yong-tao ZHONG Da-fang LIU Bing 

机构地区：[1]College of Pharmacy, Jilin University, Changchun 130021, P. R. China [2]College of Life Science, Jilin University, Changchun 130012, P. R. China [3]School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, P. R. China [4]Jilin Provincial Corps Hospital, Chinese People＇s Armed Police Forces, Changchun 130052, P. R. China

出　　处：《Chemical Research in Chinese Universities》2009年第5期673-676,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.39930180 and 30070879)

摘　　要：A simple, rapid and sensitive method for the determination of protopanaxadiol in rat plasma with ginsenoside Rh2 as internal standard was developed and validated. The analyte and internal standard were extracted from plasma with ether-dichloromethane（3：2, volume ratio） and then were analyzed by reversed-phase HPLC on a short Zorbax Extend C18 column（50 mm×2.1 mm, 3.5 μm i. d.） eluted with a mobile phase consisting of acetonitrile/methanol 0.10 mmol/L ammonium acetate（45：45：10, volume ratio） at 0.4 mL/min. Detection was performed on an Applied Biosystems Sciex API 4000 mass spectrometer set at unit resolution in the multiple reaction monitoring mode. Electrospray ionization was used for ion production. The assay method shows linear over a range of 5-2000 ng/mL and intra- and inter-day precisions over this range were 〈10.0% with accuracy ranged from 86.3% to 114.1%. The limit of detection was 500 pg/mL in the plasma. The method was successfully applied to a preclinical pharmacokinetic study of protopanaxadiol（17.5 mg/kg） administered as a single oral dose.A simple, rapid and sensitive method for the determination of protopanaxadiol in rat plasma with ginsenoside Rh2 as internal standard was developed and validated. The analyte and internal standard were extracted from plasma with ether-dichloromethane（3：2, volume ratio） and then were analyzed by reversed-phase HPLC on a short Zorbax Extend C18 column（50 mm×2.1 mm, 3.5 μm i. d.） eluted with a mobile phase consisting of acetonitrile/methanol 0.10 mmol/L ammonium acetate（45：45：10, volume ratio） at 0.4 mL/min. Detection was performed on an Applied Biosystems Sciex API 4000 mass spectrometer set at unit resolution in the multiple reaction monitoring mode. Electrospray ionization was used for ion production. The assay method shows linear over a range of 5-2000 ng/mL and intra- and inter-day precisions over this range were 〈10.0% with accuracy ranged from 86.3% to 114.1%. The limit of detection was 500 pg/mL in the plasma. The method was successfully applied to a preclinical pharmacokinetic study of protopanaxadiol（17.5 mg/kg） administered as a single oral dose.

关 键 词：PROTOPANAXADIOL Ginsenoside Rh2 LC/MS 

分 类 号：O657.72[理学—分析化学] Q51[理学—化学]