印迹细胞学检查在结膜松弛症中的应用  

Application of Impression Cytology in Conjunctivochalasis

作　　者：田晓艳[1] 艾育德[2] 谭红霞[1] 

机构地区：[1]阿拉善盟中心医院眼科,内蒙古巴彦浩特750306 [2]内蒙古医学院附属医院眼科,内蒙古呼和浩特010050

出　　处：《内蒙古医学杂志》2009年第8期901-903,共3页Inner Mongolia Medical Journal

摘　　要：目的:探讨结膜印迹细胞学检查(CIC)在结膜松弛症(CCH)发病诊断中的作用。方法:选取正常组15人30眼,结膜松弛组15人30眼,除采用泪液分泌实验I(Schirmer I)、泪膜破裂时间(BUT)、虎红染色(RBS)及荧光素染色(FLS)计分等常用眼表泪液功能检查,重点采用CIC,通过观察结膜上皮细胞情况及杯状细胞数来了解正常眼、结膜松弛眼的泪膜黏液层的情况。结果:Schirmer I试验结膜松弛症组与对照组比较,差异无显著意义(P>0.05),结膜松弛症组BUT、虎红染色、荧光素活体染色异常较对照组多,差异有显著意义(P<0.01),印迹细胞学分级及杯状细胞数组间均有显著的差异,正常组分级主要集中于0级,CCH的分级主要集中于1级和2级,杯状细胞与RBS、FLS计分呈负相关,与BUT计分呈正相关。结论:结膜印迹细胞学检查通过分级、观察杯状细胞数变化,了解正常人与结膜松弛症患者泪膜黏液层的情况。Objective： To evaluate the usefulness of impression cytology in diagnosing of conjunc- tivochalasis. Methods： In addition to the Schirmer Test I , Tear Break- up Time, Rose Bengal Staining, Fluo- rescein Staining were used, we tested the tear film mucous layer by CIC for control group and conjunctivochalasis patients . Results： The Schirrner Test I did not show a significant change（P 〉0.05）, there was significant dif- ferences in Tear Break- up Time, Rose Bengal Staining, Fluorescein Staining （P 〈 0.01）, CIC grading of the controls group revealed grade 0, for the CCH, were grade 1 and grade 2, this demonstrates a significant difference between the two groups. There is a negative correlation between goblet cell density and RBS results. Conclusion： CIC grading and goblet cell density can be used to assess the mucous layer to distinguish between normal and CCH patients.

关 键 词：结膜松弛症 印迹细胞学 杯状细胞 

分 类 号：R777[医药卫生—眼科]