油菜根圈枯草芽孢杆菌Tu-100抗真菌范围和防治作用  

Antifungal spectrum and bio-control effect of Bacillus subtilis Tu-100 on pathogenic fungi

作　　者：江木兰[1,2] 赵瑞 胡小加[1] 万霞[1] 张银波[1] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学重点开放实验室,湖北武汉430062 [3]中国国家气象局科技司,北京100081

出　　处：《中国油料作物学报》2009年第3期355-358,共4页Chinese Journal of Oil Crop Sciences

基　　金：十一五国家科技支撑计划项目(2006BAD21B03);湖北省农业创新中心项目

摘　　要：枯草芽孢杆菌（Bacillus subtilis）具有抗胁迫生长和强竞争性等优势，是一种具有应用潜力的农作物病害生防细菌。本研究以来源于油菜根际、具有促生作用的枯草芽孢杆菌Tu-100为材料，研究了其抑制农作物真菌病原菌的作用范围和效果。结果证明：Tu-100对供试的12种真菌均有明显抑制作用。对峙培养试验结果显示：直接用Tu-100的发酵菌液与供试的10种病原真菌形成了明显的抑菌圈，浓缩10倍后对供试的12种真菌均形成直径27-40mm的抑菌圈。离体叶片上防治菌核病（Sclerotinia sclerotiorum）的试验结果显示：Tu-100菌液对油菜、大豆菌核病和玉米小斑病（Bipotam maydis）均有显著防治作用。该菌株防治植物根部病害的进一步研究将具有实际应用意义。BaciUus subtilis is a biocontrol agent with a promising application potential against plant fungal diseases. This agent is highly competitive and have some advantageous characteristics such as strong growth under disadvantageous conditions. This paper reported anti - fungal spectrum and bio - control effect of Bacillus subtilis Tu - 100 that was isolated from rape rhizosphere and could promote plant growth. The results showed that LB liquid cultures of Tu - 100 had obvious antagonistic effect on 10 of tested 12 crop pathogenic fungi, forming 27 - 40 mm anti -fungal circle when 10 × Tu - 100 liquid was used. The control efficiency of Tu - 100 against Sclerotinia sclerotiorum de Bary and Bipolaris maydis was significant on detached rape, soybean and maize leaves in vitro, therefore, it has a significant importance in filed application.

关 键 词：枯草芽孢杆菌Tu-100 抗真菌范围 生防作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]