新型气体信号分子H_2S对吗啡依赖大鼠伏核及海马cAMP水平的影响  

Effect of new gaseous signal molecule-H_2S to cAMP of Accumbens and Hippocampus in morphine-dependent rats

作　　者：秦伟[1] 刘晓红[1] 肖顺武[2] 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563003 [2]遵义医学院神经外科,贵州遵义563003

出　　处：《中国现代医学杂志》2009年第17期2623-2625,共3页China Journal of Modern Medicine

基　　金：贵州省科技厅(No:20083066);遵义医学院硕士启动基金(No:F-278)

摘　　要：目的探讨新型气体信号分子硫化氢(H2S)对吗啡依赖大鼠伏核、海马cAMP含量的影响。方法将30只SD大鼠,随机分为生理盐水组、吗啡依赖组和吗啡依赖+NaHS组(每组10只)。按剂量递增原则,给SD大鼠皮下注射吗啡建立吗啡依赖大鼠模型,每天注射吗啡前30min给予吗啡依赖+NaHS组大鼠腹腔注射NaHS,其余各组注射等量的生理盐水。模型建立成功后化学分光光度法测定血浆H2S含量,放射免疫法检测伏核和海马cAMP的含量。结果依赖组大鼠血浆中H2S含量低于生理盐水组(P<0.05),伏核和海马cAMP含量均高于生理盐水组(P<0.05);吗啡依赖+NaHS组大鼠血浆中H2S含量高于吗啡依赖组(P<0.05),伏核和海马cAMP含量均低于吗啡依赖组(P<0.05),而与生理盐水组相近(P>0.05)。结论H2S可能引起吗啡依赖大鼠伏核、海马cAMP水平的变化。[Objective] To explore the effects of new gaseous signal molecule-hydrogen sulfide （H2S） to cAMP of the Aceumbens and the Hippoeampus levels in morphine-dependent rats. [Method] 30 SD rats were divided into normal saline, morphine-dependent and morphine-dependent＋NariS group （10 each group）. According to the princiele of increasing dose daily,morphine was injected by subcutaneous to establish morphine-dependent rat models, before morphine was injected 30 minutes daily, injected Naris to morphine-dependence ＋ Naris rats, after morphine-dependent rat models were established, detected H2S content of plasma by spectrophotometry and the cAMP eontent of the aeeumbens and hippocampus by RIA （radioimmunoassay）. [Result] H2S content of plasma decreased in dependent rats than the saline group （P 〈0.05）, cAMP content in the Accumbens and Hippocampus were higher than normal saline group （P 〈0.05）; H2S content of plasma increased in morphine-dependent ＋ NariS rats than morphin-dependence group （P 〈0.05）, cAMP level was lower in Aecumbens and Hippocampus than the morphine-dependent group （P 〈0.05）, while similar to the saline group （P 〉0.05）. [Conclusion] H2S may be involved in cAMP change of Aecumbens and Hippoeampus in morphine-dependent rats.

关 键 词：硫化氢 吗啡依赖 CAMP 

分 类 号：R-332[医药卫生]