苦豆子总碱舒张家兔离体主动脉的研究  

Vasorelaxational effects of total alkali Sophora alopecuroids on rabbit aorta in vitro

作　　者：张团笑[1] 牛彩琴[2] 买文丽[1] 敬华娥[1] 刘红[1] 

机构地区：[1]川北医学院基础医学院生理学教研室,四川南充637007 [2]川北医学院中西医临床医学系,四川南充637007

出　　处：《中国中药杂志》2009年第18期2379-2382,共4页China Journal of Chinese Materia Medica

基　　金：四川省教育厅重点项目(08ZA109)

摘　　要：目的：研究苦豆子总碱（total alkaliSophora alopecuroidsL,TASa）舒张家兔离体主动脉血管的作用机制。方法：采用离体恒温灌流浴槽,以去甲肾上腺素（noradrenaline,NA）预收缩后,给予TASa（5,10,20,40 mg.L-1）观察其舒张血管的量效关系;将标本分为对照组、TASa组、TASa＋吲哚美辛（1×10-5mol.L-1）、TASa＋普萘洛尔（1×10-5mol.L-1）、TASa＋左旋硝基精氨酸（1×10-4mol.L-1）和TASa＋去内皮细胞6组探讨TASa舒张血管的机制;用TASa（40 mg.L-1）温育标本后,观察TASa对NA（1×10-8～1×10-5mol.L-1）,KC l（6.3～100 mmol.L-1）和CaC l2（1×10-5～1×10-2mol.L-1）量效收缩曲线的影响。结果：TASa能舒张主动脉血管,有量效依赖性（r=0.94,P〈0.01）;去内皮、左旋硝基精氨酸、吲哚美辛和普萘洛尔对TASa舒张血管的作用无明显影响;TASa能压低NA,KC l和CaC l2的量效收缩曲线。结论：TASa可能是抑制内质网Ca2＋释放和拮抗Ca^2＋通道，降低胞浆Ca^2＋而使血管舒张。Objective： To study the vasodilation effects of the Total alkali Sophora alopecuroids L （TASa） on rabbit thoracic aortic rings in vitro and the possible mechanisms. Method： Rabbit aortic rings were isolated and precontracted with noradrenaline （NA） and then were divided into six groups including control group, TASa group, TASa ＋ 1 × 10^-5 mol · L^-1 indomethacin （Indo）, TASa ＋ 1 × 10^-5 mol · L^-1 propranolol （Prop）, TASa ＋ 1 × 10^-4 mol · L^-1 Nm-nitro-L-arginine （L-NNA）, TASa ＋ removal of endothelium. The vasodilation effects of TASa were investigated. In addition, the thoracic aortic rings were pre-treated with TASa （40 mg·L^-1） and then the thoracic aortic rings were treated with cumulative NA （1 10^-8 -1 10^-5 mol·L^-1）, KCI （6. 3-1 00 mmol·L^-1） or CaC12 （ 1 10^-5 -1 10^-2 mol·L^-1 ）. The dose response curves of aortic rings were recorded. Result： TASa can relax isolated rabbit aorta and has an obvious concentration-dependent relaxation （ r = 0. 94, P 〈 0. 01 ）. The relaxant effect of TASa was no significant reducing by removal of endothelium and by treatment with L-NNA, Indo or Prop. In addition, TASa can decrease the dose response curves of aortic rings to NA, KC1 or CaC12. Conclusion ： The vasodilation effects of TASa are related to not only inhibition of intracellular calcium release, but also reduction to calcium flow to the interior of the cell with blockage of calcium channels.

关 键 词：苦豆子总碱 离体主动脉 内皮细胞 钙离子 

分 类 号：R285.5[医药卫生—中药学] R338[医药卫生—中医学]