贵州高原湖泊细菌和病毒分布特征及影响因素  

Distribution patterns and related affecting factors of bacterial and viral abundance in lakes of Guizhou tableland.

作　　者：陆婷[1,2] 梁小兵[1] 曾佳[1,2] 张伟[1,2] 

机构地区：[1]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [2]中国科学院研究生院,北京100049

出　　处：《生态学杂志》2009年第10期1996-2001,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(40473050);国家重大基础研究项目(2006CB4003200);贵州省科学技术基金资助项目(20092040)

摘　　要：应用SYBR Green Ⅰ荧光显微镜计数法,研究了贵州阿哈湖和百花湖细菌和病毒丰度的垂直分布特征,探讨了它们与温度、pH、溶解氧(DO)、电导率和溶解有机碳(DOC)之间的关系。两湖DOC的浓度范围为1.48～2.96mg.L-1,细菌丰度为(3.32～16.6)×106个.ml-1,病毒丰度为(1.87～12.5)×107个.ml-1,病毒丰度与细菌丰度的比值(VBR)变化范围为4.09～12.77,平均值均为百花湖(BH)>阿哈湖入湖区(AHB)>阿哈湖湖心区(AHA)。细菌和病毒丰度由湖水表层至底层呈减少趋势。两湖细菌丰度均与温度、pH、DO呈显著正相关,与电导率呈显著负相关,且与病毒丰度呈极显著性正相关。阿哈湖细菌丰度与DOC显著正相关。在阿哈湖湖心区,病毒丰度与DOC呈显著正相关。在阿哈湖入湖区,病毒丰度与DOC无相关性。百花湖中,细菌、病毒丰度均与DOC无相关性。By using epifluorescence microscopy with SYBR Green I, the vertical distribution patterns of bacterial and viral abundance in Aha Lake and Baihua Lake in Guizhou Province of China were studied, with the effects of water temperature, pH, DO, conductivity, and DOC concentration analyzed. In the two lakes, the DOC concentration ranged from 1.48 to 2. 96 mg · L^-1,bacterial abundance was 3.32 × 10^6-16.6 × 10^6 cells · ml^-1 and viral abundance was 1.87 × 10^7-12.5 × 10^7 cells · ml^-1. The virus/bacteria ratio （VBR） varied from 4. 09 to 12.77, with the mean value in the sequence of Baihua Lake 〉 Aha Lake entrance 〉 central Aha Lake. The bacterial and viral abundance decreased from surface water to bottom water. In the two lakes,bacterial abundance was significantly positively correlated with water temperature, pH, DO, and viral abundance, and negatively correlated with water conductivity. There was a significant positive relationship between bacterial abundance and DOC in Aha Lake, and between viral abundance and DOC in central Aha Lake, but no significant relationships were observed between viral abundance and DOC in Aha Lake entrance, and between bacterial and viral abundance and DOC in Baihua Lake.

关 键 词：湖泊 SYBR Green I 细菌 病毒 DOC 

分 类 号：X172[环境科学与工程—环境科学]