γ-氨基丁酸对肝癌细胞株HepG-2恶性表型的影响  

Effect of γ-aminobutyric acid on malignant phenotypes of hepatocellular carcinoma cell line HepG-2

作　　者：李跃辉[1] 刘妍[1] 王甲甲[1] 胡锦跃[1] 周国华[1] 谢平丽[1] 李官成[1] 

机构地区：[1]中南大学肿瘤研究所,长沙410078

出　　处：《中南大学学报（医学版）》2009年第8期752-756,共5页Journal of Central South University ：Medical Science

摘　　要：目的:研究γ-氨基丁酸(GABA)对肝癌细胞株HepG-2增殖能力及恶性表型的影响。方法:用不同浓度的GABA与人肝癌细胞HepG-2作用后,用四甲基偶氮唑蓝(MTT)法测定细胞生长速度,并测定其倍增时间,流式细胞仪检测细胞周期的改变,软琼脂细胞集落形式实验和裸鼠成瘤实验检测细胞的恶性表型。结果:MTT实验结果表明实验组细胞的生长速度明显快于对照组细胞的生长速度,且呈剂量依赖性,其细胞周期分布也发生改变,G1期细胞减少,S期细胞增多;对照组细胞和各浓度组细胞的平均倍增时间分别为39.0,30.6,30.0,27.3,26.6和38.2 h,软琼脂细胞集落形成率依次为3.2%,4.2%,5.4%,6.6%,6.5%,3.5%,Balb/c裸鼠成瘤实验显示,对照组和实验组的肿瘤平均质量依次为0.285和1.382 g,其差异有统计学意义(P<0.01)。结论:GABA能促进细胞增殖,并引起细胞恶化改变。Objective To determine the effect of γ-aminobutyric acid（GABA） on proliferation and malignant phenotypes of hepatocellular carcinoma cell line HepG-2.Methods HepG-2 cells were cultured by routine method,and then treated with different concentrations of GABA.The proliferation of HepG-2 cells was measured through MTT,doubling time and cell cycles by flow cytometry.The malignant phenotypes were investigated by soft agar colony formation assay.Results Compared with the control group,GABA efficiently stimulated the proliferation of HepG-2 cells in a dose-dependent manner and affected the distribution of cell cycles of HepG-2 cells.The doubling time of the control group and the GABA-treated group were 39.0,30.6,30.0,27.3,26.6,and 38.2 h,respectively.The colony formation rates were 3.2%,4.2%,5.4%,6.6%,6.5%,and 3.5%,respectively.Tumorigenicity testing showed that the average weights of tumor was 1.382 g,and 0.285 g for the 2 groups.The difference between the control group and the GABA-treated groups was significant（P0.01）.Conclusion GABA can enhance the proliferation and malignant phenotypes of HepG-2 cells.

关 键 词：Γ-氨基丁酸 HEPG-2细胞 肝癌 增殖 恶性表型 

分 类 号：R735.7[医药卫生—肿瘤]