人成釉细胞瘤裸鼠皮下移植瘤的病理研究  

Study of human ameloblastoma xenograft in nude mice

作　　者：张磊涛[1] 殷学民[1] 黄洪章[2] 曾东林[2] 王建广[3] 陶谦[2] 张彬[3] 

机构地区：[1]南方医科大学南方医院口腔科,广州510515 [2]中山大学光华口腔医学院口腔颌面外科 [3]中山大学附属第二医院口腔颌面外科

出　　处：《现代口腔医学杂志》2009年第5期505-508,共4页Journal of Modern Stomatology

基　　金：国家自然科学基金(30471896);广东省自然科学基金(04300240)

摘　　要：目的观察分析人成釉细胞瘤裸鼠皮下移植瘤的病理特点。方法无菌条件下将人成釉细胞瘤组织标本切碎、过筛,接种于BALB/c-nu/nu裸小鼠前肢根部的背侧皮下近肌肉处及后背部皮下。无特殊病原体环境下饲养。观察裸鼠及移植瘤生长情况。显微镜下观察移植瘤的侵袭情况。结果裸鼠生长良好。移植瘤病理分析移植瘤的病理类型不同于原来的丛状型。肿瘤细胞散在或聚集成团片状排列,可见到非典型的类似滤泡样及颗粒样的结构,但大多数细胞排列紊乱无典型的成釉细胞瘤病理亚型。结论成功建立人成釉细胞瘤裸鼠皮下移植瘤动物模型;人成釉细胞瘤移植瘤的病理类型和移植组织的病理特点不一致,需要进一步研究。Objective To study the characters of xenografts in nude mice.Methods Fresh ameloblastoma tissues were minced into 1mm3-2mm3 size and subcutaneous transplanted into the back of the two anterior extremities root and the posterior back of nude mice. The pathological analysis was performed to study the xenografts.Results All of the xenografts were survival. There was follicular structure of ameloblastoma in the connective tissue around the xenografts. The follicular structure had the typical V-G standard. There was no follicular structure in connective tissue of the experimental side,and only some residual follicular structure scattered in connective tissue.Conclusion The subcutaneous xenotransplanted tumor model of human ameloblastoma in nude mice was successfully established. The pathological character of xenografts in nude mice was not consistant with the human ameloblastoma.

关 键 词：成釉细胞瘤 移植瘤 裸小鼠 

分 类 号：R739.8[医药卫生—肿瘤]