APCmin／＋小鼠缺失维生素D受体对肠道肿瘤生长的影响  

Effects of inactivation of vitamin D receptor in intestinal tumor growth of APCmin/＋ mice

作　　者：康红[1] 郑维[2] 喻宏[3] 

机构地区：[1]湖南省人民医院急诊科,湖南长沙410005 [2]湖南省人民医院普外科 [3]湖南省人民医院病理科

出　　处：《中国医师杂志》2009年第9期1174-1177,共4页Journal of Chinese Physician

基　　金：湖南省教育厅科研基金资助项目（07C573）;湖南省卫生厅科研基金资助项目（B2007134）;湖南省人民医院仁术重点科研基金项目（2006年）

摘　　要：目的探讨维生素D受体（VDR）失活对APCmin／＋小鼠肠道肿瘤生长的影响及机制。方法通过构建APCmin/＋VDR-／-小鼠模型（n=8），与APCmin／＋小鼠比较（n=8），4月龄时观察肠道肿瘤大小及数目情况，肿瘤作HE染色以判断病理类型。免疫组化检测肿瘤相关基因BCL-2、vimentin-1、Stat-1和MSH-2蛋白的表达。结果4月龄时2组鼠比较，APCmin／＋VDR-／-小鼠〉3mm肿瘤明显增多（P〈0．01）。HE染色显示肠道肿瘤为管状腺瘤。APCmin／＋肿瘤的Stat-1表达较强，而MSH-2和vimentin-1表达在APCmin／＋VDR-／-肿瘤中均更强。结论维生素D受体缺失促使APCmin／＋小鼠肠道肿瘤的发展。Objective To explore effects and mechanism of inactivation of vitamin D receptor （VDR） in intestinal tumor growth of APCmin/＋ mice. Methods To generate APCmin/＋ VDR -/- mice through breeding, the number and size of small intestinal and colonic tumors were assessed and compared between APCmin/＋ and APCmin/＋ VDR -/- mice. The intestinal tumors were diagnosed with HE stain. The expressions of BCL-2 ,vimentin-1 ,Stat-1 and MSH-2 proteins of tumors were determined by immunohistochemistry and compared be- tween APCmin/＋ and APCmin/＋ VDR - / - mice. Results A comparison between APCmin/＋ and APCmin/＋ VDR - / - mice revealed that the number of the tumors, which were larger than 3mm, was significantly increased in APCmin/＋ VDR -/- mice at 4 months of age （ P 〈 0. 01 ）. HE staining indicated fistulous adenomas from small intestine and colon of two groups. Immunostaining showed Stat - 1 level in APC- rain/＋ tumors were increased and MSH-2 and vimentin-1 levels in APCmin/＋ VDR -/- mice were increased, compared to APCmin/＋ tumors. Conclusion These observations suggested that inactivation of VDR promoted the intestinal tumor growth of APCmin/＋mice.

关 键 词：肠肿瘤/遗传学 受体 骨化三醇/生物合成 基因 bcl-2/生物合成 中间丝蛋白质类/生物合成 原癌基因蛋白质类/生物合成 

分 类 号：R735.2[医药卫生—肿瘤]