植物内生真菌镰刀菌对马铃薯晚疫病菌的抑制研究  

Inhibition Effect of Plant Endophytic Fusarium spp. on Phytophthora infestans

作　　者：苏珍珠[1] 罗文富[1] 杨艳丽[1] 杨子祥[1] 

机构地区：[1]云南农业大学云南省植物病理重点实验室,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2009年第5期667-671,683,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省科技攻关项目(2002NG209)

摘　　要：鉴定了分离自7种植物上的11株内生真菌镰刀菌并研究了它们对马铃薯晚疫病菌的抑菌活性。对峙培养试验表明:11株内生真菌镰刀菌对该病菌均有抑制作用,其中菌株e060-1,e060-3,e060-4的抑制率达50%以上。这些内生真菌镰刀菌的毒素粗提液对该病菌菌丝生长有更明显的抑制作用,其中e012-3,e060-4和e032毒素粗提液的抑菌率达100%。毒素粗提液能明显抑制晚疫病菌孢子对离体叶片的侵染,其中菌株e032毒素粗提液的抑制率达97.1%。Eleven strains of endophytic Fusarium spp. isolated from 7 kinds of healthy plants were i- dentified. And the inhibitory activities of these endophytic Fusarium spp. against Phytophthora infestans were tested. All these strains showed a certain degree inhibitory effects on the hypha growth of P. infestans by dual culture. The inhibition rates of strains e060 - 1, e060 - 3 and e060 - 4 were above 50%. The inhibitory effects of unrefined toxin extracts obtained from endophytic Fusarium spp. were more significant against hypha growth of P. infestans. The inhibitory rates of unrefined toxin extracts of e012 -3, e060 -4 and e032 were up to 100%. The unrefined toxin extracts of endophytic Fusarium also inhibited the infection of P. infestans spore to detached leaves. The unrefined toxin extract of strain e032 had the best inhibitory effects at the inhibitory rate of 97.1%.

关 键 词：内生真菌镰刀菌 晚疫病菌 抑菌活性 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]