1,3,7,9-四甲基尿酸抗抑郁作用的实验研究  

Experimental study of theacrine on antidepressant effects

作　　者：谢果[1,2] 吴敏芝[2,3] 黄映如[2] 曹雅婷[2] 李来东[2] 周合樑[1] 朱瑞[2] 廖燕峰[2] 栗原博[1] 

机构地区：[1]暨南大学中药及天然药物研究所,广东广州510632 [2]电子科技大学中山学院,广东中山528402 [3]南方医科大学药学院,广东广州510515

出　　处：《中国药理学通报》2009年第9期1160-1163,共4页Chinese Pharmacological Bulletin

基　　金：国家科技支撑计划资助项目(No2006BAI06A00)

摘　　要：目的探讨1,3,7,9-四甲基尿酸(theacrine,TC)的抗抑郁作用。方法采用悬尾实验、强迫游泳实验、自主活动实验、育亨宾(yohimbine)毒性实验、利血平(reserpine)实验以及5-羟色胺酸(5-HTP)诱导小鼠甩头行为等动物模型(各组剂量分别为3、10及30mg·kg-1,bid,连续7d灌胃给药)来评价TC的抗实验性抑郁效果及其可能的作用机制。结果与空白对照组相比,TC能明显缩短小鼠悬尾不动时间(P<0.01)及增加强迫游泳小鼠拨动转轮旋转次数(P<0.05),且对自主活动无影响。TC可一定程度上增强yohim-bine诱发实验动物死亡率,增强5-HTP导致的甩头行为(P<0.01)。此外,TC也具有明显地改善reserpine引起的小鼠体温下降(P<0.01),运动不能(P<0.05)和眼脸下垂(P<0.01)的作用。结论TC在多种抑郁模型上显示一定的药理活性,这些作用可能与影响单胺类神经递质有关。Aim To investigate the antidepressant effects of theacrine （TC）. Methods The antidepressant effects of TC were examined by a series of experiments including tail suspension test, forced swimming test, ambulatory activity test, yohimbine induced toxicity test, reserpine test and 5-HTP induced head-twitching test （The doses of groups were 3,10,30 mg·kg^-1, bid respectively for continued 7 days of intragastric administration）. Results Compared with the control group, TC could effectively shorten the immobility time in the tail suspension test （P 〈 0. 05 ） and enhance the rotatory locomotor activities during forced swimming test （P 〈0. 05）, but didn＇t affect the ambulatory activity. Moreover, TC also enhanced the mortality induced by yohimbine, and increased the head-twitching number induced by 5-HTP （P 〈 0. 01 ）. In addition, TC could markedly improve such symptoms as hypothermia （P 〈 0. 01 ）, akinesia （P 〈 0.05 ）, and eye ptosis of mice （P 〈0.01 ） induced by reserpine. Conclusions TC has antidepressant effects in various depression models of mice, which may be contributed to its influence on monoamine neurotransmitter.

关 键 词：苦茶 1 3 7 9-四甲基尿酸 抑郁 行为绝望 育享宾 5-羟色胺酸 利血平 

分 类 号：R-332[医药卫生] R284.1