三羟异黄酮抑制大鼠脑缺血铜蓝蛋白的时相变化  

Genistein inhibits the time coursed expression changes of ceruloplasmin in rats of focal cerebral ischemia

作　　者：马慧娟[1] 王立轩[2] 马会杰[1] 王慧娟[2] 武宇明[1] 李陈莉[2] 

机构地区：[1]河北医科大学生理学教研室,河北石家庄050017 [2]河北医科大学组织胚胎学教研室,河北石家庄050017

出　　处：《中国药理学通报》2009年第9期1164-1167,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30400513);河北省自然科学基金资助项目(No303467)

摘　　要：目的探讨植物性雌激素三羟异黄酮(genistein,GST)对局灶性永久性脑缺血♂大鼠的大脑皮层铜蓝蛋白(cerulo-plasmin,CP)表达的影响。方法利用免疫组织化学和Western blot法,观察颈内动脉注射GST对♂大鼠局灶性永久性脑缺血模型中大脑皮层CP表达的影响。结果脑缺血过程中CP的表达呈时相性变化,缺血6、12、24h低于假手术组,48、72h高于假手术组;GST组CP的表达在不同时段均明显低于缺血组,雌激素受体阻断剂他莫昔芬(tamox-ifen,TAM)可阻断此作用。结论GST可抑制脑缺血时CP的时相性变化,提示GST可通过雌激素受体抑制脑内铁相关的氧化应激损伤,提供神经保护作用。Aim To define the effect of genistein （GST） on ceruloplasmin （CP） expression in cerebral cortex of focal cerebral ischemia male rats. Methods The effect of GST on CP expression were investigated by means of immunohistochemistry and Western blot in cerebral cortex of focal cerebral ischemia male rats. Results The expression of CP was time coursed during brain ischemia. According to the sham group, CP expression decreased in 6 h-, 12 h- and 24 b-ischemia groups, and then increased gradually in 48 h- and 72 h-ischemia rats. GST weakened the ischemia-induced CP change. Pretreatment with an antiestrogen tamoxifen （TAM） could completely abolish the effect of GST. Conclusion The present results indicate that GST may inhibit the time coursed expression changes of CP and then provide neuroprotective effects by inhibiting iron related oxidative stress during brain ischemia via estrogen receptor.

关 键 词：三羟异黄酮 铜蓝蛋白 他莫昔芬 脑缺血 大脑皮层 神经保护 

分 类 号：R-332[医药卫生] R322.81