ERK1/2抑制剂PD98059对大肠癌细胞侵袭和转移的影响  

Effect of PD98059 on invasion and metastasis of human colon cancer cells

作　　者：周燕红[1] 刘毅飞[1] 王瑶芬[1] 

机构地区：[1]咸宁学院医学院,湖北咸宁437100

出　　处：《山东医药》2009年第34期4-5,共2页Shandong Medical Journal

基　　金：湖北省卫生厅科研基金指导性项目(JX3C11)

摘　　要：目的观察细胞外信号调节蛋白激酶(ERK1/2)抑制剂PD98059对大肠癌细胞侵袭和转移的影响,探讨其可能机制。方法PD98059不同浓度(0、10、20、40μmol/L)干预大肠癌细胞24 h。用Boyden小室检测其体外运动和侵袭能力,Western blot法检测ERK1/2磷酸化蛋白和ERK1/2蛋白表达情况。结果随着PD98059剂量增大,大肠癌细胞穿膜数逐渐减少(P<0.05),磷酸化ERK1/2蛋白水平逐渐降低(P<0.05),ERK1/2蛋白水平无明显变化(P>0.05)。结论PD98059能抑制大肠癌细胞的运动和侵袭力;其机制与PD98059抑制磷酸化ERK1/2有关。Objective To investigate the effect and mechanism of PD98059 on invasion and metastasis of human colon cancer cells. Methods Colon cancer cells were cultured with different concentration of PD98059 （0 μmol/L,10 μmol/L, 20 μmol/L,40 μmol/L,a ERK1/2 specific inhibitor） for 24 hours. The mobility and invasion of colon cancer cells were measured by boyden chamber assay in vitro. The phosphorylation levels of extracellular signal regulated kinase （ERK1/2） were detected by Western blot method. Results The mobility of LOVO cells was inhibited by PD98059 in adose-depended manners. The decreases of tyrosine phosphorylatinn of ERK1/2 in LOVO were dose dependence of PD98059. Conclusions PD98059 could inhibit mobility and invasion of human colon cancer cells. The decrease of p-ERK1/2 may be a possible mechanism .

关 键 词：肠肿瘤 侵袭 转移 细胞外信号调节蛋白激酶 PD98059 

分 类 号：R574.6[医药卫生—消化系统]