冷冻联合粒巨细胞集落刺激因子对前列腺癌肺转移的防治作用  

Combined cryoablation with GM-CSF treatment to prevent lung metastasis of prostate cancer

作　　者：司同国[1] 郭志[1] 王海涛[1] 韩艳萍[1] 郝希山[1] 

机构地区：[1]天津医科大学附属肿瘤医院介入治疗科天津市肿瘤防治重点实验室,300060

出　　处：《中华实验外科杂志》2009年第10期1284-1286,共3页Chinese Journal of Experimental Surgery

基　　金：天津市自然科学基金资助项目（09JCYBJC10400）

摘　　要：目的观察冷冻联合粒巨细胞集落刺激因子（GM—CSF）治疗前列腺癌前后机体抗肿瘤免疫反应，及其对肺转移瘤的影响。方法复制前列腺癌皮下移植瘤。将动物模型随机分为4组：A组（单纯冷冻组）、B组（冷冻联合GM—CSF组）、C组（GM—CSF组）、D组（对照组）。动态观察治疗前、治疗后7、14、21d肿瘤体积、肺转移瘤发生率。分离脾脏淋巴细胞，酶联免疫斑点（ELIS—POT）试验检测分泌肿瘤特异性干扰素（IFN）-γ的Th细胞数。乳酸脱氢酶（LDH）释放试验检测细胞毒性T淋巴细胞（CTL）肿瘤特异性杀伤活性。结果治疗后21d，A组、B组、C组、D组平均肿瘤体积分别是1．22、0．26、2．69、2．70cm^3；肺转移瘤发生率分别是：3／5、0／5、3／5、4／5；每1×10^5个脾细胞中肿瘤特异性IFN-γ^＋Th细胞数分别是：35．80±1．48、239．00±1．58、16．40±1．14、14．80±0．83；CTL特异杀伤活性分别为：（8．54±0．11）％、（44．50±0．85）％、（8．04±0．18）％、（6．92±0．41）％。结论冷冻联合GM—CSF可明显增强机体抗肿瘤特异性免疫反应，有效防治肺转移瘤。Objective To assess the anti-tumor immune response after combined cryoablation with GM-CSF treatment for prostate cancer,and the effect on lung metastasis. Methods Mouse model of prostate cancer was used, and the tumor-bearing mice were divided into four groups ： group A （ cryoablation treatment） ,group B （combined cryoabaltion and GM-CSF treatment）, group C （GM-CSF treatment）, group D （control group）. Gross tumor volume,and the rate of lung metastasis were assessed on the posttreatment day 0,7,14,21. Lymphocytes of spleen were isolated, and the number of tumor-specific IFN-γ＋ Th cells after treatment was counted by the method of enzyme linked immunospot assay （ELISPOT）. And tumor-specific cytolytie activity of cytotoxic T lymphocyte （CTL） was measured by LDH assay. Results On the post-treatment day 21, the gross tumor volume in groups A, B, C, and D was 1.22,0.26,2.69, and 2.70 cm^3 ,the rate of lung metastasis was 3/5,0/5,3/5, and 4/5, the number of IFN-γ^＋ cells every 1 × 10^5 Th ceils was 35.80 ± 1.48,239.00 ± 1.58,16.40 ± 1.14, and 14.80 ± 0.83,cytolytic activity of CTL against prostate cancer cells was （ 8.54 ± 0.11 ） % , （44.50 ± 0.85 ） % , （ 8.04 ± 0.18 ） %, and （ 6.92 ± 0.41 ）% respectively. Conclusion Combined cryoablation with GM-CSF treatment could increase tumorspecific immune response, and prevent lung metastasis effectively.

关 键 词：前列腺肿瘤 转移 冷冻消融 免疫反应 

分 类 号：R737.25[医药卫生—肿瘤]