CDC25A蛋白和mRNA在胆管癌中的表达及其意义  

The expression and significance of CDC25A protein and gene in cholangioncarcinoma

作　　者：路文彦[1] 刘三光[1] 边伟[1] 张桂东[1] 杨蒙[1] 

机构地区：[1]河北医科大学第二医院肝胆外科,石家庄050000

出　　处：《中华实验外科杂志》2009年第10期1332-1334,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测胆管癌和癌旁0．5cm胆管组织及手术切缘的正常胆管组织中CDC25A蛋白及mRNA的表达，探讨其在胆管癌发生发展中的作用。方法应用流式细胞分析术检测58例胆管癌和正常胆管组织中CDC25A蛋白的表达，同时应用逆转录一聚合酶链反应（RT-PCR）技术检测44例术中所取的新鲜的胆管癌、同个体癌旁胆管及其手术切缘正常胆管组织中CDC25AmRNA的表达，并与临床资料进行相关分析。结果正常胆管组织、癌旁组织、癌组织中CDC25AmRNA相对表达量分别为0．3832±0．1440、0．4550±0．1744、0．5802±0．4188，CDC25AmRNA在三组之间表达呈上升趋势（P〈0．05）。CDC25A蛋白表达趋势同其相对应的基因表达趋势一致，即CDC25A的mRNA在胆管癌组织中高表达（P〈0．05）。结论CDC25A基因转录和蛋白的表达可能参与了胆管癌的发生发展过程，检测CDC25A的表达可能有助于阐明胆管癌的发生发展机制。Objective To detect the express of CDC25A protein and gene in cholangioncarcinoma and to elucidate its role. Methods The express of CDC25A protein was detected by FCM in 58 eases of cholangioncarcinoma and normal bile duct, and the express of CDC25A gene was detected in 44 cases of cholangioncarcinoma,adjacent to cholangioncarcinoma and normal bile duct by RT-PCR. The correlation was analysed statistically between the expression of CDC25A protein and mRNA and clinicopathological parameters in cholangioncarcinoma. Results The express of CDC25A mRNA in normal bile duct, adjacent to cholangioncarcinoma, cholangioncarcinoma respectively was 0. 3832± 0. 1440, 0. 4550 ± 0. 1744, 0. 5802±0. 4188 ,The express of CDC25A mRNA was higher in cholangioncarcinoma than others （P 〈 0.05 ）. The protein of CDC25A in eholangioncarcinoma was higher than normal bile duet （ P 〈 0.05 ）. Conclusion cdc25a gene transcription and protein expression is mostly like involved in the proliferation and malignant transformation of bile epithelial and then malignant progression of bile duct carcinomas. The detection of cdc25a expression may elucidate the carcinogenesis of bile duct.

关 键 词：胆管癌 CDC25A 流式细胞分析术(FCM) RT—PCR 

分 类 号：R735.8[医药卫生—肿瘤] R735.7[医药卫生—临床医学]