长瓣短柱茶种子油等在2BS细胞培养中的延缓衰老效应  

Comparison of Antisenile Effects of Seed Oil of Camellia grijsii and Certain Other Oil from Woody Crops on 2BS Cell Culture

作　　者：吴开云[1,2] 翁月霞 费学谦[1,2] 杨婉琴 孙雪忠[1,2] 

机构地区：[1]中国林业科学研究院亚热带林业研究所 [2]浙江省富阳市林业局

出　　处：《林业科学研究》1998年第4期355-360,共6页Forest Research

基　　金：国家自然科学基金;浙江省自然科学基金

摘　　要：将长瓣短柱茶种子油及其它木本食油经酶解处理后，以适当比例加入ＭＥＭ培养液培养人胚肺成纤维细胞（２ＢＳ）。测定各油组细胞膜过氧化脂质（ＬＰＯ）水平、细胞培养传代数、细胞生长速度变化、细胞贴壁能力和细胞外观形态变化。结果表明长瓣短柱茶种子油具有与橄榄油相当的延缓细胞衰老效果，其第４６代细胞的细胞膜过氧化脂质的积累显著低于普通油茶油、长瓣短柱茶油非皂化成分、三油酸甘油脂组。在加入橄榄油或长瓣短柱茶种子油酶解产物组中培养的２ＢＳ细胞比加入普通茶油酶解产物组的细胞多传２代寿命。初步认为在以油酸为主要构成的食用油中亚油酸等多种不饱和脂肪酸成分以适当比例存在对延长细胞活力有重要意义；长瓣短柱茶油非皂化成分组在各项测试中仅次于长瓣短柱茶油与橄榄油组而依次优于普通茶油、三油酸甘油脂加长瓣短柱茶油非皂化成分和三油酸甘油脂组，提示该部分中含有一定的延缓衰老活性成分。The seed oil of Camellia grijsii (GRI) and C.oleifera (OLF), olive oil (OLV) and olein (OLN) after enzymolisis etc.were added to MEM medium to culture the human diploid foetal lung fibroblasts(2BS). The results showed the similar antisenile effects of the seed oil of C.grijsii and olive oil. Significant lower accumulation of LPO in cell membrane tested at 46th generation of the above two groups than that of OLF,unsaponifiable fraction of GRI(NSG),OLN+NSG and OLN groups were found. The lifespan of cells in GRI and OLV groups wast 2 generations longer than that in OLF group. The comparison of morphology of 2BS under microscope revealed the same sequence as the result of LPO testing and lifespan observation. Preliminary conclusion is that an appropriate existence of linoleic acid is essential to prolong the lifespan of 2BS with the main component of oleic acid in certain edible oils,and there are some active components in unsaponifiable fraction of GRI which could delay the aging process of 2BS.

关 键 词：长瓣短柱茶 种子油 人胚细胞培养 延缓衰老效应 

分 类 号：S794.401[农业科学—林木遗传育种] S565.901[农业科学—林学]