CD44v6、p27表达与大肠癌临床病理学因素的关系  

Expression and clinicopathologic significance of COn4v6 and p27 in colorectal carcinomas

作　　者：胡大为[1] 敖亚洲[1] 杨景哲[2] 王立平[3] 马丽辉[1] 王助新[1] 

机构地区：[1]承德医学院附属医院肿瘤外科二病区,067000 [2]承德医学院附属医院烧伤整形科,067000 [3]承德医学院附属医院普外科二病区,067000

出　　处：《肿瘤研究与临床》2009年第9期597-599,共3页Cancer Research and Clinic

摘　　要：目的探讨CD44v6和p27在大肠癌中的表达及其临床意义。方法对50例手术切除的大肠癌新鲜标本及相应的正常大肠黏膜，应用流式细胞术（FCM）检测CD44v6的表达；同步制备上述病例大肠癌组织石蜡标本，采用免疫组织化学方法检测大肠癌组织中p27的表达，结合病理及临床资料进行统计学分析。结果CD44v6和p27在大肠癌组织及正常黏膜组织中表达存在明显差异（P〈0．01），而且CD44v6和p27的表达与大肠癌的分化程度、有无淋巴结转移、有无远端器官转移、Duke分期及预后相关（P值均〈0．05），CD44v6。的表达与大肠癌的浸润深度无明显相关（P〉0．05），但p27的表达与大肠癌的浸润深度明显相关（P〈0．05）。结论CD44v6和p27的表达与大肠癌的临床病理学特征密切相关，两者在大肠癌组织中的表达呈一定的负相关，同时检测二者对判断大肠癌的恶性程度、复发转移潜能，评价患者的预后及术后选择合理的治疗方案具有一定的参考价值。Objective To observe the expression and reveal clinical significance of CD44v6 and p27 in colorectal carcinoma. Methods Collecting 50 cases of fresh specimen of coloreetal carcinoma and normal mucous from excision operation. CD44v6 expression was detected by means of FCM in 50 cases of eolorectal carcinoma and corresponding normal mucous, p27 protein was studied by SP immunohistoehemical method. Analysis of statistics was performed by combining clinical and patholoical materials. Results CD44v6 and p27 expression in colorectal carcinoma was significantly higher than that in normal mucous （P 〈0.01）. As well as the expression of CD44v6 and p27 was obviously associated with differentiated grade, lymph node metastasis, distant metastasis and Dukes stage （P 〈0.05）. The expression of CD44v6 was not evidently correlated with the depth of tumor invasion（P 〉0.05）, but the expression of p27 was correlated with the depth of tumor invasion（P 〈0.05）. Conclusion The expression of CD44v6 and p27 is markedly related with clinicopathological characters of colorectal carcinoma.CD44v6 expression and p27 expression are obvious negative relationship in colorectal carcinoma. There is a certain value of reference for assessing the malignant grade and the potential of recurrence and metastasis,evaluating patient prognosis and selecting rational treatment after operation.

关 键 词：肠肿瘤 流式细胞术 免疫组化 抗原 CD44V6 P27 

分 类 号：R735.34[医药卫生—肿瘤]