金地鼠颊囊癌前病变的细胞增殖动力学研究  

Flow cytometry dynamic study binding to bromodeoxyuridine in golden hamster cheek pouch premalignancy.

作　　者：周永梅[1] 曹宏康[1] 周曾同[1] 徐宏波[1] 吴直江 高奇蓉 龚忠萍 

机构地区：[1]上海第二医科大学口腔医学院口腔内科教研室

出　　处：《上海口腔医学》1998年第3期140-142,共3页Shanghai Journal of Stomatology

摘　　要：目的研究口腔上皮组织癌和癌前病变的细胞动力学变化特征。方法采用Ｓａｌｅｙ的ＤＭＢＡ涂布诱导法，建立金地鼠颊囊癌前病变模型，并用溴脱氧尿苷流式细胞动力学检测方法，对３０只叙利亚种金地鼠进行分组对照研究。结果涂药组光镜下的细胞病变程度随涂药时间延长而逐渐加重，而空白对照组无变化；ＦＣＭ检测显示涂药组与对照组在Ｓ期阳性细胞检出率存在显著差异。即使在光镜下表现为轻度不典型增生时，ＦＣＭ检测结果也有显著差别。Objective In order to study cellular dynamicfeatures in carcinoma and premalignancy originated inepithelial tissue.Methods The golden hamster pouch premalignant model with dimethylbenzanthracence (DMBA) smeared was established by Salley and detected with flow cytometry (FCM) binding to bromodeoxyuridine (BRDU), 30 Syrian golden hamsters were grouped and done contrast study.Results The cellular lesions were aggravated gradually along with extention of smeared DMBA microstopically.FCM detective results demonstrated that the detective rate of Sporiod cell between contrast and smeared group had significant difference.Even though mild dysplsia presented microscopically FCM detective result and significant difference.Conclusion The study suggests that golden hamster cheek pouch premalignancy has cellular dynamicfeatures of dysplasia.

关 键 词：金地鼠 溴脱氧尿苷 流式细胞动力学 口腔肿瘤 

分 类 号：R739.8[医药卫生—肿瘤]