大鼠创伤性脑损伤肝早期细胞凋亡和肿瘤坏死因子-α的变化及意义  

The Changes and Effect of Hepatocyte Apoptosis and TNF-α at Early Stage after Traumatic Brain Injury in Rats

作　　者：王晓[1] 朱玉群[1] 王天懿[1] 王燕斌[1] 杨昭徐[1] 

机构地区：[1]首都医科大学附属北京天坛医院消化内科,100050

出　　处：《湖南中医药大学学报》2009年第7期14-16,共3页Journal of Hunan University of Chinese Medicine

摘　　要：目的:探讨大鼠创伤性脑损伤(TBI)肝早期细胞凋亡和肿瘤坏死因子-α(TNF-α)的变化及与应激性肝损害(HSI)的关系。方法:用改良Allen法建立TBI模型。40只健康雄性Wister大鼠随机分为5组,正常对照组和TBI后6、12、24、48h时相组。测定各组大鼠血清ALT、AST和TNF-α水平,采用流式细胞术检测肝早期细胞凋亡率,透射电镜观察肝脏早期凋亡细胞,并观察肝组织超微结构的改变。结果:TBI早期,各组血清ALT、AST和TNF-α水平升高,与对照组比较差异显著;早期细胞凋亡率于伤后6h增多并达峰值。TBI后各组光镜和电镜可观察到肝组织不同程度受损。结论:TBI后肝早期细胞凋亡和TNF-α增加可能是继发HSI的重要机制之一。Objective： To explore the changes of hepatocyte apoptosis and tumor necrosis factor-α（TNF-α）at early stage after traumatic brain injury（TBI）in rats and the effect on hepatic stress injury（HSl）. Methods： The animal model was built by using an improved Allen＇ s method.Forty healthy male Wistar rats had been divided randomly into five groups：control group,TBI group which was divided into four subgroups according to different time：6h, 12h,24h and 48h after acute brain trauma.The serum levels of ALT, AST and TNF-α were measured.The hepatocyte apoptosis at early stage was detected through transmission electron microscope and flow cytometer.And pathological changes of liver tissue under electron microscope were observed. Results： At early period of TBI the serum levels of ALT, AST and TNF-α were increased in TBI group,which were significantly different compared with control group.The rate of apoptosis got to a peak at 6h after TBI.Injuries of liver tissue at different degrees were observed in TBI group. Conclusion： Apoptosis at early stage and TNF-α may be involved in the pathogenesis of HSI which occur at early period after TBI.

关 键 词：创伤性脑损伤 细胞凋亡 肿瘤坏死因子-Α 应激性肝损害 

分 类 号：R-332[医药卫生]