Nogo-A在脑缺血再灌注白质损伤中的表达及其意义  

The expression of Nogo-A after cerebral ischemia-reperfusion injury and its significance

作　　者：高晓玉[1] 王得新[2] 张拥波[2] 

机构地区：[1]烟台毓璜顶医院神经内科,山东烟台264000 [2]首都医科大学附属北京友谊医院

出　　处：《中国老年学杂志》2009年第18期2323-2325,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.30570626)

摘　　要：目的检测脑缺血再灌注后不同时间点轴突生长抑制因子Nogo-A在损伤白质区的基因表达,探讨Nogo-A与缺血再灌注白质损伤的可能关系。方法制备大鼠大脑中动脉缺血再灌注(MCAO)模型,实时定量聚合酶链反应方法(Relative Quantification PCR,RQ-PCR)检测各时间点Nogo-A在损伤白质区域的基因表达。结果在损伤白质区域,Nogo-A基因表达在缺血再灌注1d时表达量开始减少(与假手术组相比,P<0.05),7d时降至最低,14～21d表达量开始上调,21d时仍低于假手术组水平(与假手术组相比,P<0.001)。结论缺血再灌注损伤后白质区域的Nogo-A基因表达呈现动态变化规律,针对Nogo-A进行干预有望为缺血再灌注损伤后的脑保护治疗找到新的靶点。Objective To study the expression of Nogo-A in cerebral white matter after focal cerebral ischemia-reperfusion（I/R） in rats, and explore the relationship between Nogo-A and cerebral white matter lesions after ischemia. Methods Sprague-Dawley male rats were subjected to 2 h of middle cerebral artery occlusion （MCAO）. Relative quantification PCR（RQ-PCR） was performed to examine the expressions of Nogo-A gene at different reperfusion time. Results The expression level of Nogo-A gene in the white matter was decreased in 1 d group（P 〈0.05 vs sham group） ,and was the weakest in 7 d group. It was increased in 14 -21 d group, and in 21 d the expression level was still lower than that of sham group （ P 〈 0. 001 ）. Conclusions Nogo-A expreesion has dynamic changes in the white matter after focal cerebral I/R. Nogo-A could be as one of the targets for neuroprotective therapy after cerebral ischemia.

关 键 词：脑缺血 Nogo—A 白质损伤 

分 类 号：R743.32[医药卫生—神经病学与精神病学]